Evaluation of a conserved HA274-288 epitope to detect antibodies to highly pathogenic avian influenza virus H5N1 in Indonesian commercial poultry.

Evaluation of a conserved HA274-288 epitope to detect antibodies to highly pathogenic avian influenza virus H5N1 in Indonesian commercial poultry. Avian Pathol. 2016 Mar 24;:1-45 Authors: Wawegama NK, Tarigan S, Indriani R, Selleck P, Adjid RA, Syafriati T, Hardiman, Durr PA, Ignjatovic J Abstract A peptide ELISA based on an epitope in the haemagglutinin (HA) of avian influenza virus H5N1, amino acid positions 274 -288 (HA274-288) was evaluated for detection of H5N1 specific antibodies. An optimised ELISA based on the tetrameric form of the HA274-288 epitope designated MP15 gave low background with non-immune chicken sera and detected vaccinated and infected birds. The HA274-288 epitope was highly conserved in Indonesian H5N1 strains and antibody responses were detected in the majority of the vaccinated chickens regardless of the H5N1 strain used for vaccination. The HA274-288 epitope was also conserved in the majority of H5N1 strain from the neighbouring Asian region, and other H5 subtypes potentially allowing for a wider use of the MP15 ELISA in H5N1 vaccinated and infected flocks. The MP15 ELISA results correlated significantly with haemagglutination inhibition (HI) test results and test sensitivity and specificity were 87% and 92%, respectively. The MP15 ELISA titres were significantly higher than the HI titres in all immune sera allowing for sera to be tested at a single dilution of 1:400 which is of advantage in routine surveil...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research