Detection and molecular typing of human adenovirus associated with respiratory illness in Kerala.

Detection and molecular typing of human adenovirus associated with respiratory illness in Kerala. Jpn J Infect Dis. 2016 Mar 18; Authors: Thounaojam AD, Balakrishnan A, Mun AB Abstract Adenoviruses are responsible for approximately 5-10% of acute respiratory infections globally. However, there are limited reports on circulating respiratory human adenovirus (HAdV) types in India. We detected HAdV in the post mortem specimens of a young child died due to acute febrile illness. In order to know the circulating adenovirus types in the Alappuzha region, retrospectively, the samples (n=235) collected from influenza-like illness patients for influenza surveillance program were screened for HAdV. Fourteen samples were positive for adenovirus by PCR. Adenovirus was isolated from three out of fourteen PCR positive samples in HEK 293 cell lines. The viral strains isolated in the study were from children between ages 6 and 10 years. The isolates were identified as adenovirus species C and E. Further, the sequencing of fiber gene and BLAST search revealed that the two of the isolates were type HAdV-C2 and the third isolate was a HAdV-E4. Fiber gene sequence based phylogenetic tree showed that the isolate HAdV-E4 closed to the Japanese HAdV-E4 strain, whereas the isolates HAdV-C2 formed a distinct cluster. Although respiratory infections due to HAdV-E4 are generally seen in adults, this is the first instance showing the involvement of HAdV-E4 stra...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research