Genetic diversity in Streptococcus dysgalactiae subsp. equisimilis isolates from patients with invasive and noninvasive infections in a Japanese university hospital (2014-2015).

Genetic diversity in Streptococcus dysgalactiae subsp. equisimilis isolates from patients with invasive and noninvasive infections in a Japanese university hospital (2014-2015). Jpn J Infect Dis. 2016 Mar 18; Authors: Fujita T, Horiuchi A, Ogawa M, Yoshida H, Hirose Y, Nagano N, Takahashi T Abstract Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates with β-hemolysis and carbohydrate groups G/C are recovered increasingly from invasive infections in Japan. The aim of this study was to determine epidemiological characteristics of SDSE isolates circulating locally among patients with invasive and noninvasive infections. We selected groups G/C β-hemolytic streptococci from a database at the Clinical Laboratory of Kitasato University Medical Center from May 2014 through April 2015. Thirteen isolates were identified as SDSE based on the data from API-20 Strep and 16S rRNA sequencing. The samples were from seven sterile specimens (blood) and six non-sterile specimens (pus/sputum/vaginal secretion). Information about the patients with invasive or noninvasive SDSE infections was retrieved from their medical charts. We performed emm genotyping, multilocus sequence typing, dendrogram analysis of the samples using pulse-field gel electrophoresis (PFGE), and amplifications of streptococcal inhibitor of a complement-mediated cell lysis-like gene (sicG)/ antimicrobial resistance determinants. We identified eight different emm genotypes,...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research