Measuring the intra-renal distribution of glomerular volumes from histologic sections.

Measuring the intra-renal distribution of glomerular volumes from histologic sections. Am J Physiol Renal Physiol. 2016 Mar 16;:ajprenal.00382.2015 Authors: Hann BD, Baldelomar EJ, Charlton JR, Bennett KM Abstract Glomerular volume is an important metric reflecting glomerular filtration surface area within the kidney. Glomerular hypertrophy, or increased glomerular volume, may be an important marker for renal stress. Current stereologic techniques report the average glomerular volume (AVglom) within the kidney. These techniques cannot assess the spatial or regional heterogeneity common in developing renal pathology. Here, we report a novel "Unfolding" technique to measure the actual distribution of individual glomerular volumes from the 2D glomerular profiles observed by optical microscopy. The Unfolding technique was first developed and tested for accuracy with simulations, then applied to measure the number of glomeruli Nglom, AVglom, and the intra-renal distribution of individual glomerular volume (IVglom) in the oligosyndactyl (Os/(+)) mouse model compared to wild type (WT) controls. The Os/(+) mice had fewer, larger glomeruli than WT mice: Nglom was 12,126±1,658 (glomeruli/kidney) in the WT mice and 5,516±899 in the Os/(+) mice; AVglom was 2.01±0.28 x10(-4) mm(3) for the WT mice and 3.47±0.35 x10(-4) mm(3) for the Os/(+) mice. Comparing the volume distributions in Os/(+) and WT kidneys, we observed that the Os/(+) distributi...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research