Abstract A33: Expression of BRCA2 and Mir-21 in sporadic and BRCA2 mutated breast cancer in Iceland

The BRCA2 protein plays an important role in the repair of double-stranded DNA breaks (DSBs) by homologous recombination. An inherited founder mutation in the BRCA2 gene accounts for 6-7 % of all breast cancers in Iceland. Breast cancer in BRCA2 mutation carriers is associated with poor long-term prognosis. It has been reported that > 20% of sporadic breast cancers display a BRCA-like phenotype (BRCA‘ness), suggesting a defect in the DSB repair pathway. Little is known about the role of miRNAs in BRCA2 related breast cancer. Analysis of array CGH data from BRCA2 breast tumors shows that the mir-21 locus on chromosome 17 is amplified in > 70% of BRCA2 breast tumors. Mir-21 is a known oncomir with known target genes involved in tumor suppression, amongst others. High expression of mir-21 has been associated with poor-prognosis in other breast cancer populations. The aim of the study is to investigate the level and variation of BRCA2 and mir-21 expression in breast tumors from BRCA2 mutation carriers and breast tumors from non-carriers. Furthermore we want to see if there is association between BRCA2 and mir-21 expression.Expression analysis was performed by qPCR on RNA extracted from fresh frozen tumor tissue from BRCA2 mutation carriers and sporadic breast tumors. Expression of mir-21 was analyzed using specific mir-21 Taqman probes, with RNU48 as an endogenous control. Expression of BRCA2 was analyzed using gene specific primers detected by SYBR® Green, with H...
Source: Molecular Cancer Research - Category: Cancer & Oncology Authors: Tags: Genomics - Sporadic and Hereditary: Poster Presentations - Proffered Abstracts Source Type: research