Increased expression of Solute carrier family 12 member 5 via gene amplification contributes to tumour progression and metastasis and associates with poor survival in colorectal cancer

Objective Using whole genome sequencing, we identified gene amplification of solute carrier family 12 member 5 (SLC12A5) located at 20q13.12 in colorectal cancer (CRC). We analysed its amplification, overexpression, biological effects and prognostic significance in CRC. Design SLC12A5 amplification status was evaluated by fluorescence in situ hybridisation (FISH). The effects of SLC12A5 re-expression or knockdown were determined in proliferation, apoptosis, invasion and metastasis assays. SLC12A5 target genes and related pathways were identified by reporter activity and cDNA microarray analyses. Clinical impact of SLC12A5 overexpression was assessed in 195 patients with CRC. Results Amplification of SLC12A5 was verified in 78 out of 191 (40.8%) patients with primary CRC by FISH, which was positively correlated with its protein overexpression (p
Source: Gut - Category: Gastroenterology Authors: Tags: Open access, Colon cancer Source Type: research

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Background For the past 30 years, there has been a growing emphasis on communication and self-evaluation skills training in graduate medical education. This is reflected in the Next Accreditation System. The Objective Structured Clinical Examination (OSCE) is widely used in graduate medical education for assessing dimensions of interpersonal communication and counseling skills. The OSCEs may be developed to target challenging clinical scenarios difficult to capture in clinical practice and can be used as a medium for resident self-evaluation. Objectives The aims of the study were to evaluate residents' interpersonal, ...
Source: Simulation in Healthcare: The Journal of the Society for Simulation in Healthcare - Category: Medical Devices Tags: Empirical Investigations Source Type: research
A study finds similarities between cells that heal wounds and those that lead deadly cancerous invasions.
Source: The Scientist - Category: Science Tags: Notebook Magazine Issue Source Type: news
We report the genome-wide effects of KAP1 loss on the transcriptome, the chromatin state, and on recruitment of various components of the transcription machinery in the colon colorectal cancer cell line HCT116.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Other Homo sapiens Source Type: research
Journal of Medicinal ChemistryDOI: 10.1021/acs.jmedchem.0c00045
Source: Journal of Medicinal Chemistry - Category: Chemistry Authors: Source Type: research
rreira Angela Relógio Malfunctions of circadian clock trigger abnormal cellular processes and influence tumorigenesis. Using an in vitro and in vivo xenograft model, we show that circadian clock disruption via the downregulation of the core-clock genes BMAL1, PER2, and NR1D1 impacts the circadian phenotype of MYC, WEE1, and TP53, and affects proliferation, apoptosis, and cell migration. In particular, both our in vitro and in vivo results suggest an impairment of cell motility and a reduction in micrometastasis formation upon knockdown of NR1D1, accompanied by altered expression levels of SNAI1 and CD44. Inter...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
In this study, they compare two sequencing methods and design a bioinformatic analysis to establish the basis of a wide study in the research of early detection markers of colon cancer.
Source: EurekAlert! - Cancer - Category: Cancer & Oncology Source Type: news
scu Berindan-Neagoe An increasing number of studies suggest the implication of microRNAs (miRNAs) in colorectal (CRC) carcinogenesis and disease progression. Nevertheless, the basic mechanism is not yet clear. We determined plasma miRNA expression levels using Agilent microarray technology followed by overlapping with The Cancer Genome Atlas (TCGA) tissue data and a qRT-PCR validation step and analysis of the altered miRNA signatures to emphasize new mechanistic insights. For TGCA dataset, we identified 156 altered miRNAs (79 downregulated and 77 upregulated) in colorectal tissue samples versus normal tissue. The mi...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: British Journal of Cancer - Category: Cancer & Oncology Authors: Source Type: research
Contributors : Dimitrios Iliopoulos ; Marina KoutsioumpaSeries Type : Non-coding RNA profiling by array ; Expression profiling by arrayOrganism : Homo sapiensTranscriptional lncRNAs expression comparing human colon cancer tissues to normal colon tissues using Agilent-062918 Human lncRNA array V4.0.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by array Expression profiling by array Homo sapiens Source Type: research
Journal of Natural ProductsDOI: 10.1021/acs.jnatprod.0c00003
Source: Journal of Natural Products - Category: Biochemistry Authors: Source Type: research
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