Fluoroquinolone and Macrolide Resistance in Campylobacter Jejuni Isolated from Broiler Slaughterhouses in Southern Brazil.

Fluoroquinolone and Macrolide Resistance in Campylobacter Jejuni Isolated from Broiler Slaughterhouses in Southern Brazil. Avian Pathol. 2016 Feb 29;:1-22 Authors: Sierra-Arguello YM, Perdoncini G, B Morgan R, P Salle CT, S Moraes HL, P Gomes MJ, Nascimento VP Abstract Campylobacter jejuni became recognized as a leading cause of acute bacterial gastroenteritis in humans. The over-use of antimicrobials in the human population and in animal husbandry has led to an increase in antimicrobial-resistant infections, particularly with fluoroquinolones and macrolides. The aim of the present study was to provide information of the current status of antimicrobial resistance patterns in C. jejuni from poultry sources. Fifty strains were recovered from broiler slaughterhouses in Rio Grande do Sul state, Brazil, 2012. The strains were investigated for antimicrobial susceptibility against three agents (ciprofloxacin, nalidixic acid and erythromycin) by minimal inhibitory concentrations. The strains were analyzed by PCR-RFLP for detection of the Thr-86 mutation that confers resistance to ciprofloxacin. In addition, all the strains were tested for the presence of efflux systems (cmeB gene) conferring antimicrobial resistance. The MICs results showed that 98% of isolates were sensitive to erythromycin and most isolates were resistant to ciprofloxacin (94%) and nalidixic acid (90%). A complete correlation was observed between the MICs and PCR-RFLP assa...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research