In Vivo Influence ìf in Vitro Up-Regulated Genes in the Virulence of an Apec Strain Associated with Swollen Head Syndrome.

In Vivo Influence ìf in Vitro Up-Regulated Genes in the Virulence of an Apec Strain Associated with Swollen Head Syndrome. Avian Pathol. 2016 Feb 29;:1-40 Authors: de Paiva JB, Pilatti Mendes da Silva L, Casas MR, Conceição RA, Nakazato G, de Pace F, Sperandio V, da Silveira WD Abstract Avian Pathogenic Escherichia coli (APEC) is responsible for significant economic losses in the poultry industry by causing a range of systemic or localized diseases collectively termed colibacillosis. The virulence mechanisms of these strains that are pathogenic in poultry and possibly pathogenic in humans have not yet been fully elucidated. This work was developed to study if over-expressed genes in a microarray assay could be potentially involved in the pathogenicity of an APEC strain isolated from a swollen head syndrome case. For this study, five over-expressed genes were selected for the construction of null mutants [flgE (flagellar hook), tyrR (transcriptional regulator), potF (putrescine transporter), yehD (putative adhesin), and bfr (bacterioferritin)]. The constructed mutants were evaluated for their capacity for the adhesion and invasion of in vitro cultured cells, their motility capacity, and their pathogenic potential in one-day-old chickens compared with the wild-type strain (WT). The Δbfr strain showed a decreased adhesion capacity on avian fibroblasts compared with WT, in the presence and absence of alpha-D-mannopyranoside, and the ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research