Application of a Clinical Whole-Transcriptome Assay for Staging and Prognosis of Prostate Cancer Diagnosed in Needle Core Biopsy Specimens

Molecular and genomic analysis of microscopic quantities of tumor from formalin-fixed, paraffin-embedded biopsy specimens has many unique challenges. Herein, we evaluated the feasibility of obtaining transcriptome-wide RNA expression to measure prognostic classifiers in diagnostic prostate needle core biopsy specimens. One-hundred fifty-eight samples from diagnostic needle core biopsy specimens (Bx) and radical prostatectomies (RPs) were collected from 33 patients at three hospitals; each patient provided up to six tumor and benign samples.

Source: Journal of Molecular Diagnostics - March 2, 2016 Category: Pathology Authors: Beatrice S. Knudsen, Hyung L. Kim, Nicholas Erho, Heesun Shin, Mohammed Alshalalfa, Lucia L.C. Lam, Imelda Tenggara, Karen Chadwich, Theo Van Der Kwast, Neil Fleshner, Elai Davicioni, Peter R. Carroll, Matthew R. Cooperberg, June M. Chan, Jeffry P. Simko Tags: Regular Article Source Type: research