Lab-created sperm breeds healthy mice, raising hopes for end to male infertility

Scientists hail landmark achievement, but warn that artificial human sperm is a distant prospect fraught with concerns over safety, ethics and legalityA handful of healthy mice made from sperm cells created in the lab have been hailed as a milestone in research that could ultimately provide new treatments for male infertility.Scientists in China created the mice by fertilising normal mouse eggs with early-stage sperm cells that were manufactured from embryonic stem cells plucked from the animals. Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Stem cells Science Fertility problems Biology Reproduction Medical research Health Source Type: news

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Conclusion Material and MethodsTheca cells, collected from normal cycling and PCOS patient underwent ovariectomy, were seeded on culture dishes pre-coated with the extracellular matrix at a density of 6 × 104 cells/ml and cultured for 24 hours. Cells were then treated with either 3T3 conditioned media as a control (C) or Mesenchymal stem cells conditioned media (CM). The C and CM were prepared through collecting the serum-free supernatant of 3T3 and hMSCs at an optimal confluence, 48 hours later. The mRNA and protein expression of CYP17A1, CYP11A1, and DENND1A.V2 were quantified by RT-PCR and Western blot, while ...
Source: Cytotherapy - Category: Cytology Source Type: research
Xenotransplantation of spermatogonial stem cells (SSCs) has become a popular topic in various research fields because manipulating these cells can provide insights into the mechanisms associated with germ cell...
Source: Stem Cell Research and Therapy - Category: Stem Cells Authors: Tags: Research Source Type: research
Abstract In clinical infertility practice, one intractable problem is low (or absent) ovarian reserve which in turn reflects the natural oocyte depletion associated with advancing maternal age. The number of available eggs has been generally thought to be finite and strictly limited, an entrenched and largely unchallenged tenet dating back more than 50 years. In the past decade, it has been suggested that renewable ovarian germline stem cells (GSCs) exist in adults, and that such cells may be utilized as an oocyte source for women seeking to extend fertility. Currently, the issue of whether mammalian females posse...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research
In this study, the expression profiles of FGSCs and spermatogonial stem cells (SSCs) were analyzed to further reveal their similarity and difference, a nd AKT3 was predicted as a pivotal molecule for FGSCs self‐renewal. Then, we demonstrated that CDH22 interacted with PI3K to phosphorylate AKT3 and subsequently enhanced the expression levels of N‐myc and cyclin family in FGSCs to promote self‐renewal. Moreover, glial cell line‐derived neuro trophic factor (GDNF) was identified as an essential factor for FGSCs self‐renewal with a more complicated mechanism: GDNF‐GFRA1 activates AKT3 via PI3K or Src family kinase...
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research
Gonadal impairment represents an important late effect occurring after hematopoietic stem cell transplantation (HSCT), affecting the quality of life of young transplanted patients. The proportion and severity of gonadal late impairment depends on type and cumulative dosage of gonadotoxic chemotherapeutic agents, type of HSCT, age at HSCT, additional use of Total Body Irradiation (TBI) for conditioning or radiotherapy (cranial and pelvic radiotherapy) administered before HSCT [1]. Gonadal complications are recognized as a consequence of myeloablative conditioning regimens, in particular based on TBI or Busulfan (Bu), and in...
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
Conclusions Two, Not Mutually Exclusive, Hypotheses We have reviewed and organized the literature with the intent of showing the existing parallels between excessive fat accumulation and the aging process. We have categorized these reports following what have been proposed to be the nine hallmarks of aging (21) (Figure 1). Based on the evidence, two distinct hypotheses can be proposed. One is that the cellular responses provoked by an excess of nutrients cause obesity, and that obesity is responsible for accelerating the pace of aging. Supporting this hypothesis are the observations that knocking out the fat-specific ins...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Conclusion and Perspectives The ARE has been studied for a long time, and about 20 ARE-BPs have been identified since discovery of first ARE-BP, AUF1 (Brewer, 1991; Garcia-Maurino et al., 2017). The specific target mRNAs for different ARE-BPs, as well as their molecular functions on these mRNAs, and contribution of this regulation to specific biological processes are gradually being uncovered. However, with a few exceptions, the molecular mechanisms used by ARE-BPs to regulate their targets are still unknown. In particular, the mechanism to recognize and control specific targets from the large number of transcripts t...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Abstract Spermatogenesis is initiated when spermatogonial stem cells (SSCs) in the mature testes enter mitosis and trigger differentiation. Thus, spermatogenesis and the ability to maintain a continuous source of spermatogonia relies on the ability to differentiate SSCs. Many studies around the world have been performed to investigate the etiology of male infertility and recent studies have focused on the presence and identification of biomarkers. CD133 and CD24 are stem cell markers locating in the testis and spermatogonia. The aim of this study was to investigate the relationship of the CD133 and CD24 genes with...
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research
This study demonstrates GDNF signal as a pivotal molecule for self ‐renewal of FGSCs, reveals the connection of CDH22 signal pathway with GDNF signal pathway in FGSCs, and verifies AKT3 is the pivotal molecule of this signal network.© AlphaMed Press 2019
Source: Stem Cells - Category: Stem Cells Authors: Tags: Tissue ‐Specific Stem Cells Source Type: research
Xiao Wang and Haja N. Kadarmideen* Quantitative Genomics, Bioinformatics and Computational Biology Group, Department of Applied Mathematics and Computer Science, Technical University of Denmark, Kongens Lyngby, Denmark Epigenetic changes are important for understanding complex trait variation and inheritance in pigs that are also a valuable biomedical model for human health research. Testis is the main organ for reproduction and boar taint in pigs; however, there have been no studies to-date on adult pig testis epigenome. The main objective of this study was to establish a genome-wide DNA methylation map of pig t...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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