Immunological and biochemical characterizations of Coxsackievirus A6 and A10 viral particles.

In this study, CV-A6 and CV-A10 were individually cultured in rhabdomyosarcoma (RD) cells grown in medium containing serum, harvested and concentrated. In viral downstream purification, two viral fractions were separated by sucrose gradient zonal ultracentrifugation and detected using a SDS-PAGE analysis and a virus infectivity assay. These two viral fractions were formalin-inactivated, and only the infectious particle fraction was found to be capable of inducing CV-A serotype-specific neutralizing antibody responses in animal immunogenicity studies. These mouse and rabbit antisera also failed to cross-neutralize EV-A71 and CV-A16 infections. Only a combination of formalin-inactivated EV-A71, CV-A6, CV-A10 and CV-A16 multivalent vaccine candidates elicited cross-neutralizing antibody responses in both mouse and rabbit immunogenicity studies. The current results certainly provide important information for multivalent HFMD vaccine development.
PMID: 26899790 [PubMed - as supplied by publisher]

Source: Antiviral Research - February 17, 2016 Category: Virology Authors: Liu CC, Guo MS, Wu SR, Lin HY, Yang YT, Liu WC, Chow YH, Shieh DB, Wang JR, Chong P Tags: Antiviral Res Source Type: research