Antimicrobial activity of peptides derived from enzymatic hydrolysis of goat milk caseins

In this study, peptide fragments were produced from goat milk casein by proteolytic enzymes trypsin and ficin and combination of both enzymes, and their antimicrobial activity was investigated. After enzymatic treatments, antimicrobial activity against both Gram-positive and Gram-negative bacteria was enhanced. The hydrolysates were passed through ultrafiltration. The obtained hydrolysate by ficin (molecular weight (MW)
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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Publication date: Available online 16 August 2019Source: Molecular and Cellular ProbesAuthor(s): Ping Zhou, Guoyang Xie, Taobo Liang, Bei Yu, Zoraida Aguilar, Hengyi XuAbstractEmetic Bacillus cereus is one of the causative agents of foodborne diseases which can cause vomiting-type food poisoning after ingestion of contaminated food. To minimize B. cereus food poisoning, propidium monoazide (PMA) combined with quantitative polymerase chain reaction (qPCR) called PMA-qPCR was applied for detecting viable emetic B. cereus in milk. The cereulide synthetase gene of emetic B. cereus (cesB) was chosen for the primer, and PMA trea...
Source: Molecular and Cellular Probes - Category: Molecular Biology Source Type: research
ConclusionThe microfractionation protocol developed in this study enabled time-efficient screening of many plants species, using a small quantity of sample material. In addition, microfractionation served as a guiding tool for identifying individual antimicrobial compounds. Through this process, flavonoids were isolated from Derris scandens, out of which dalpanithin and vicenin-3 showed activity in the low micromolar range. The high hit rate for in vitro antibacterial properties from this ethnopharmacologically guided sample collection gives credence to the Sri Lankan traditional herbal medicine as a source for drug discov...
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 12 August 2019Source: LWTAuthor(s): Saritha Gopal Pandit, Krishna-Prashanth Ramesh Mekala, Mohankumari H. Puttananjaiah, Mohan Appasaheb DhaleAbstractFungus producing red pigment was identified as Talaromyces purpureogenus CFRM02. Agriculture by-product, bengal gram husk (BegH) was used as substrate for fermentation. Red pigment characteristics were confirmed by CIELAB and UV–vis spectrum. T. purpureogenus CFRM02 produced 0.565 Absorption Unit-AU mL−1 of red pigment. Addition of peptone, glucose and trace elements to culture medium significantly improved (≈4 fold) the pi...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: September 2019Source: Food Packaging and Shelf Life, Volume 21Author(s): Kowsalya E, MosaChristas K, Balashanmugam P, Tamil Selvi A, Jaquline Chinna Rani IAbstractNanotechnology is expected to transform many areas of food science as there is a growing demand for increased fresh food shelf life as well as the need of protection against foodborne diseases, thus, urging for the development of antimicrobial food packaging using the polymer embedded metal nanoparticles. In the present study, the stable silver nanoparticles (AgNPs) were synthesized by a green route using Vitis vinifera (black grapes) fruit peel...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research
In this study, a biphasic edible film based on sago starch (SS) and guar gum (GG) was developed, and thereafter, two essential oils (EOs), namely, carvacrol (0.75% w/w) and citral (1.0% w/w) were impregnated either individually or in combination into the blend. The morphology, optical, structural, and water barrier properties of the films were evaluated by various analytical techniques. Scanning electron micrographs exhibited the roughness on the top surface of the EO incorporated blend films, whereas the confocal microscopy confirmed the dispersibility of EOs into the blend. The tensile strength of films significantly red...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research
Molecules, Vol. 24, Pages 2701: Liquid and Vapour Phase of Lavandin (Lavandula × intermedia) Essential Oil: Chemical Composition and Antimicrobial Activity Molecules doi: 10.3390/molecules24152701 Authors: Stefania Garzoli Giovanni Turchetti Pierluigi Giacomello Antonio Tiezzi Valentina Laghezza Masci Elisa Ovidi Essential oils from Lavandula genus and the obtained hybrids are widely used for different purposes such as perfume production in the cosmetic field and for its biological properties. This is the first study on the liquid and vapour phase of Lavandula × intermedia “Gross...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Publication date: January 2020Source: Food Control, Volume 107Author(s): Irshaan Syed, Pratik Banerjee, Preetam SarkarAbstractApplication of plant-based essential oils as antimicrobial agents has received considerable attention. However, these antimicrobial compounds undergo quick depletion due to various factors such as volatile nature, low-water solubility, and interactions with lipophilic food components. Therefore, to retain the antimicrobial efficacy of such compounds, the combination with an oil-in-water based emulsion system as a carrier can be a viable option. The current work focuses on the development of geraniol...
Source: Food Control - Category: Food Science Source Type: research
CONCLUSIONS: Incorporation of chitosan hydrogel into honey can substantially enhance its antimicrobial and wound healing effects. Chitosan-hydrogel/honey (1:3) is an optimal wound dressing formulation with ample antimicrobial and healing properties. PMID: 31298661 [PubMed - as supplied by publisher]
Source: Wounds - Category: General Medicine Tags: Wounds Source Type: research
Publication date: 15 November 2019Source: Journal of Molecular Structure, Volume 1196Author(s): S. Jayakumar, D. Mahendiran, D. Arumai Selvan, A. Kalilur RahimanAbstractThe C-centered bis(imidazol-1-yl)methane-based heteroscorpionate metal(II) complexes of the type [M(L1−3)Cl] (1–9), where M = Mn(II), Ni(II) or Cu(II) have been synthesized using the heteroscorpionate ligands, (2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL1), (4-diethylamino-2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL2) and (5-bromo-2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL3). The spectral and theoretical studies suggested tetrahedral ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Abstract Two new meroditerpene pyrones, chevalone F (1) and 11-hydroxychevalone E (2), a new tryptoquivaline analog, tryptoquivaline V (3) and a new brasiliamide analog, brasiliamide G (4), together with thirteen known compounds, chevalones A-C (5-7), chevalone E (8), 11-hydroxychevalone C (9), pyripyropene A (10), isochaetominine C (11), pyrrolobenzoxazine terpenoids CJ-12662 (12) and CJ-12663 (13), fischerindoline (14), eurochevalierine (15), 1,4-diacetyl-2,5-dibenzylpiperazine-3,7''-oxide (16) and lecanorin (17) were isolated from the fungus Neosartorya pseudofischeri. Their structures were established on the b...
Source: Fitoterapia - Category: Biochemistry Authors: Tags: Fitoterapia Source Type: research
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