Resistant Starch Alters Gut Microbiome and Metabolomics Profiles Concurrent with Amelioration of Chronic Kidney Disease in Rats.

Resistant Starch Alters Gut Microbiome and Metabolomics Profiles Concurrent with Amelioration of Chronic Kidney Disease in Rats. Am J Physiol Renal Physiol. 2016 Feb 3;:ajprenal.00513.2015 Authors: Kieffer DA, Piccolo BD, Vaziri ND, Liu S, Lau WL, Khazaeli M, Nazertehrani S, Moore ME, Marco ML, Martin RJ, Adams SH Abstract Patients and animals with chronic kidney disease (CKD) exhibit profound alterations in the gut environment including shifts in microbial composition, increased fecal pH, and increased blood levels of gut microbe-derived metabolites (xeno-metabolites). The fermentable dietary fiber-high amylose maize resistant starch type 2 (HAMRS2)-has been shown to alter the gut milieu, and in CKD rat models leads to markedly improved kidney function. The aim of the current study was to identify specific cecal bacteria and cecal, blood, and urinary metabolites that associate with changes in kidney function, in order to identify potential mechanisms involved with CKD amelioration in response to dietary resistant starch. Male Sprague-Dawley rats with adenine induced CKD were fed a semi-purified low fiber or a high fiber diet (59% w/w of HAMRS2) for 3 weeks (n=9/grp). The cecal microbiome was characterized, and cecal contents, serum, and urine metabolites were analyzed. HAMRS2-fed rats displayed decreased cecal pH, decreased microbial diversity, and an increased Bacteroidetes to Firmicutes ratio. Several URS solutes were altered in t...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research