Characterisation of carbapenem resistance mechanisms and integrons in Pseudomonas aeruginosa strains from blood samples in a French hospital.

In this study, 61% of the IRPA isolates showed a multi-resistance phenotype. The MBL phenotype, detected in 3 isolates (5.4%), was linked to blaVIM-2 gene. The oprD gene was amplified in 55 IRPA (98.2%), and variations were observed in 54 of them. Insertion sequences (IS) truncating oprD was detected in 8 IRPA, being the new ISPa56 (GenBank KR258747) identified in two strains. Class 1 integrons were detected in 24 IRPA (42.9%). The blaVIM-2 gene was found inside the class 1 integron arrangements. The new integrons In1054 (intI1-aacA56-qacEΔ1-sul1) and In1160 (intI1-aacA4-aacC1d-ISKpn4-gcuE-qacEΔ1-sul1) were firstly described in this study (GenBank KM201605 and KR184824). A high clonal diversity was found in our strains. Among the variety of STs found, ST175, ST233, ST235, ST244 and ST654 were noteworthy as epidemic clones. To sum up 5.4% of IRPA showed a MBL phenotype, linked to blaVIM-2 gene. The identified oprD high polymorphism could be implicated in the variable resistance to carbapenems in IRPA. The high-risk clones dissemination is a cause of concern. PMID: 26838942 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research