Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.

Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells. Avian Pathol. 2016 Jan 26;:1-24 Authors: Kamble NM, Jawale CV, Lee JH Abstract Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid (FT). Attenuated strains of SG are proven and widely used vaccine candidates because of its advantages like induction of strong humoral and cell mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived DCs (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an MOI of 10. JOL1355 was able to invade DCs; however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355 stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Based on these res...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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This article updates our laboratory's systematic search for antigens using bioinformatics tools to clarify the Mtb H37Rv Rv3632 protein's topology and location. This article reports a C-terminal region consisting of peptides 39255 and 39256 (81Thr-Arg114) having high specific binding regarding two infection-related cell lines (A549 and U937); they inhibited mycobacterial entry to U937 cells in a concentration-dependent manner. Rv3632 forms part of the mycobacterial cell envelope, formed by six linear synthetic peptides. Circular dichroism enabled determining the protein's secondary structure. It was also found that peptide...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
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Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
This study was undertaken to compare the expression of interferon-gamma (IFN-γ) in chickens generated by different vaccine formulations. Eighty day-old Lohmann layer chicks were divided into four groups of twenty birds each for the experiment. This comprised a non-vaccinated negative control group (NEG), a group vaccinated with the live 9R vaccine by the injection route (SC), a group vaccinated with alginate-coated chitosan microparticles encapsulating live plasmid-cured S. Gallinarum strain 9 (PC) by the oral route and a group vaccinated with a weak attenuated live S. Gallinarum strain 9 encapsulated in alginate-coa...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractFowl typhoid (FT), a septicemic disease caused bySalmonella Gallinarum (SG), and H9N2 influenza infection are two economically important diseases that affect poultry industry worldwide. Herein, we exploited a live attenuated SG mutant (JOL967) to deliver highly conserved extracellular domains of H9N2 M2 (M2e) to induce protective immunity against both H9N2 infection and FT. To increase the immunogenicity of M2e, we physically linked it with CD40L and cloned the fusion gene into either prokaryotic constitutive expression vector pJHL65 or mammalian expression vector pcDNA3.1+. Then pJHL65-M2eCD40L or pcDNA-M2eCD40L r...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
AbstractFowl typhoid (FT), a septicemic disease caused bySalmonella Gallinarum (SG), and infectious bronchitis (IB) are two economically important avian diseases that affect poultry industry worldwide. Herein, we exploited a live attenuated SG mutant, JOL967, to deliver spike (S) protein 1 of IB virus (V) to elicit protective immunity against both FT and IB in chickens. The codon optimized S1 nucleotide sequence was cloned in-frame into a prokaryotic constitutive expression vector, pJHL65. Subsequently, empty pJHL65 or recombinant pJHL65-S1 plasmid was electroporated into JOL967 and the resultant clones were designated as ...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
Salmonella enterica infection affects a wide range of animals and humans, and a small number of serovars cause typhoid-like infections, one characteristic of which is persistent infection in convalescents. Avian-specific S. enterica serovar Pullorum produces systemic disease in young chickens, which is followed by a carrier state in convalescent birds, leading to infection of the ovary at sexual maturity and vertical transmission. However, the immunological basis of persistent infection remains unclear. S. enterica serovar Enteritidis is taxonomically closely related but does not show this characteristic. Differences in th...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Bacterial Infections Source Type: research
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Source: Veterinary Research - Category: Veterinary Research Source Type: research
Abstract Soluble plantain (Musa paradisiaca) nonstarch polysaccharides (NSPs) have previously been shown to prevent pathogenic interaction with the intestinal epithelium. Here, we examined whether plantain NSP could prevent the invasion of the intestinal mucosa by Salmonella enterica Gallinarum, a causative agent of fowl typhoid. In vitro assays using B1OXI cells were performed with monolayers pretreated with/without plantain NSP, before inoculation with Salm. Gallinarum 287/91. Chicks were fed from hatch on a pellet diet containing 50 mg day−1 plantain NSP, followed by oral inoculation with Salm. ...
Source: Letters in Applied Microbiology - Category: Microbiology Authors: Tags: Original Article Source Type: research
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