Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.

Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells. Avian Pathol. 2016 Jan 26;:1-24 Authors: Kamble NM, Jawale CV, Lee JH Abstract Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid (FT). Attenuated strains of SG are proven and widely used vaccine candidates because of its advantages like induction of strong humoral and cell mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived DCs (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an MOI of 10. JOL1355 was able to invade DCs; however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355 stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Ba...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research