Isolation and detection of duck astrovirus CPH: implications for epidemiology and pathogenicity.

Isolation and detection of duck astrovirus CPH: implications for epidemiology and pathogenicity. Avian Pathol. 2016 Jan 26;:1-28 Authors: Liu N, Jiang M, Wang M, Wang F, Zhang B, Zhang D Abstract The transmission routes of duck astrovirus CPH (DAstV/CPH) and its pathogenicity in duck embryos were investigated. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) developed in this study, DAstV/CPH was detected in 23/50 fresh droppings of breeder ducks, 39/65 breeding eggs, 26/31 dead embryos and 6/10 newly hatched ducklings, which were taken from a Pekin duck farm where DAstV/CPH had previously been identified. This finding, and the detection of DAstV/CPH in 36/130 dead-in-shell duck embryo samples collected from different hatcheries located in six provinces, suggests that the virus may be horizontally and vertically transmitted and associated with hatchability problems. Inoculation and repeated passages in embryonating duck eggs resulted in isolation of DAstV/CPH. The virus caused severe chorioallantoic membrane lesions as well as growth retardation and embryo mortality, indicating that DAstV/CPH is pathogenic for duck embryos. The effect of DAstV/CPH on hatching was confirmed by an embryo infection experiment in which 8/10 9-day-old duck embryos inoculated with the third passage of DAstV/CPH were unable to hatch, with most embryos succumbing in the final stage of incubation. The use of RT-PCR on the hatched ducklings provided evidence that t...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
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Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
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Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
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Source: Virology Journal - Category: Virology Authors: Tags: Short report Source Type: research
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Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
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Source: Viruses - Category: Virology Authors: Tags: Review Source Type: research
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Source: Archives of Virology - Category: Virology Source Type: research
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