Endothelial-to-Mesenchymal Transition and endothelial cilia in EPC-mediated postischemic kidney protection.

Endothelial-to-Mesenchymal Transition and endothelial cilia in EPC-mediated postischemic kidney protection. Am J Physiol Renal Physiol. 2016 Jan 20;:ajprenal.00306.2015 Authors: Patschan D, Schwarze K, Henze E, Patschan S, Müller GA Abstract Renal ischemia induces peritubular capillary rarefication and fibrosis, the latter partly resulting from Endothelial-to-Mesenchymal transition (EndoMT). Endothelial cilia transmit blood flow-associated forces into the cell. Early EPCs (eEPCs) have been shown to protect mice from AKI in the short-term. Aim of the study was to analyze mid-term consequences of eEPC treatment in the context of endothelial cilia and EndoMT. Male C57/Bl6N mice were subjected to unilateral renal ischemia post-uninephrectomy. Syngeneic murine eEPCs were systemically injected at the time of reperfusion. Animals were investigated 1, 4, and 6 weeks later. Cultured mature endothelial cells were exposed to a variable flow with versus without eEPC supernatant incubation. Systemically injected eEPCs reduced serum creatinine levels at weeks 1 (35 and 45 minutes) and 4 (45 minutes). Interstitial fibrosis was significantly diminished by cell treatment at all time points as well, EndoMT was less pronounced at week 4 (35 minutes) and 6 (45 minutes). eEPC supernatant reduced aSMA expression and alpha-tubulin abundances in flow-treated cultured mature endothelial cells while percentages of cilium+ cells increased. The loss of peritub...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research