Chromogenic nitrophenolate-based substrates for light-driven hybrid P450 BM3 enzyme assay.

Chromogenic nitrophenolate-based substrates for light-driven hybrid P450 BM3 enzyme assay.
J Inorg Biochem. 2015 Dec 10;
Authors: Lam Q, Cortez A, Nguyen TT, Kato M, Cheruzel L
Abstract
The incorporation of a p-nitrophenoxy moiety in substrates has enabled the development of colorimetric assays to rapidly screen for O-demethylation activity of P450 enzymes. For the light-driven hybrid P450 BM3 enzymes, where a Ru(II) photosensitizer powers the enzyme upon visible light irradiation, we have investigated a family of p-nitrophenoxy derivatives as useful chromogenic substrates compatible with the light-driven approach. The validation of this assay and its adaptability to a 96-well plate format will enable the screening of the next generation of hybrid P450 BM3 enzymes towards C-H bond functionalization of non-natural substrates.
PMID: 26712653 [PubMed - as supplied by publisher]

Source: Journal of Inorganic Biochemistry - December 10, 2015 Category: Biochemistry Authors: Lam Q, Cortez A, Nguyen TT, Kato M, Cheruzel L Tags: J Inorg Biochem Source Type: research