A Multiplexed Amplicon Approach for Detecting Gene Fusions by Next-Generation Sequencing

We describe a next-generation sequencing approach that uses a multiplex PCR-based amplicon panel to interrogate fusion transcripts that involve 19 driver genes and 94 partners implicated in solid tumors. The panel also includes control assays that evaluate the 3′/5′ expression ratios of 12 oncogenic kinases, which might be used to infer gene fusion events when the partner is unknown or not included on the panel.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Technical Advance Source Type: research