HIV-1 Capsid Stabilization Assay

The stability of the HIV-1 core in the cytoplasm is crucial for productive HIV-1 infection. Mutations that stabilize or destabilize the core showed defects in HIV-1 reverse transcription and infection. We developed a novel and simple assay to measure stability of in vitro-assembled HIV-1 CA-NC complexes. This assay allowed us to demonstrate that cytosolic extracts strongly stabilize the HIV-1 core (Fricke et al., J Virol 87:10587–10597, 2013). By using our novel assay, one can measure the ability of different drugs to modulate the stability of in vitro-assembled HIV-1 CA-NC complexes, such as PF74, CAP-1, IXN-053, cyclosporine A, Bi2, and the peptide CAI. We also found that purified CPSF6 (1-321) protein stabilizes in vitro-assembled HIV-1 CA-NC complexes (Fricke et al., J Virol 87:10587–10597, 2013). Here we describe in detail the use of this capsid stability assay. We believe that our assay can be a powerful tool to assess HIV-1 capsid stability in vitro.
Source: Springer protocols feed by Immunology - Category: Allergy & Immunology Source Type: news

Related Links:

Authors: PMID: 32493135 [PubMed - in process]
Source: Tumori - Category: Cancer & Oncology Tags: Tumori Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s):
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s):
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s):
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s): Gilead Amit
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s):
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s):
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s): Chelsea Whyte
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s): Adam Vaughan
Source: New Scientist - Category: Science Source Type: research
Publication date: 6 June 2020Source: New Scientist, Volume 246, Issue 3285Author(s): Jim Peebles
Source: New Scientist - Category: Science Source Type: research
More News: Allergy & Immunology | Restasis