Α ten year surveillance study of carbapenemase-producing Klebsiella pneumoniae in a tertiary care Greek University hospital: predominance of KPC over VIM or NDM-producing isolates.

Α ten year surveillance study of carbapenemase-producing Klebsiella pneumoniae in a tertiary care Greek University hospital: predominance of KPC over VIM or NDM-producing isolates. J Med Microbiol. 2015 Dec 23; Authors: Spyropoulou A, Papadimitriou-Olivgeris M, Bartzavali C, Vamvakopoulou S, Marangos M, Spiliopoulou I, Anastassiou ED, Christofidou M Abstract Resistance patterns and carbapenemase gene presence among K. pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant specimens were identified by the Vitek 2 Advanced Expert System. Antibiotic susceptibility testing was performed by the agar disk diffusion method and Etest. The strains were tested for the presence of blaVIM, blaIMP, blaKPC, blaNDM and blaOXA-48 genes by PCR. PFGE of chromosomal Xbal DNA digests was performed. A total of 3449 K. pneumoniae isolates were recovered during the last decade. Among them, 1668 (48%) were carbapenemase-producing; 1333 (80%) KPC, 286 (17%) VIM, 45 (3%) KPC and VIM, and four NDM-producing. Their resistance rates to gentamicin, colistin and tigecycline was 41%, 23% and 16%, respectively. VIM- K. pneumoniae were isolated in 2005 and since 2008 were endemic. KPC-K. pneumoniae were introduced in 2008 and till now represent the predominant carbapenemase-...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research