Vacuolar myopathic lesion in a Holstein-Friesian dairy cow affected with bovine dilated cardiomyopathy: a preliminary report

Abstract Occurrence of vacuolar myopathy caused by factors other than inherited glycogen storage condition is uncommon in domestic animals. This preliminary report describes the detection of a vacuolar myopathic lesion in a 13-year-old Holstein- Friesian dairy cow which showed an 8-day history of cardiac disease. Gross inspection after slaughter revealed edema in a variety of tissues, dilatation and hypertrophy of the cardiac ventricles, and hepatic congestion. Histopathology revealed degenerative myocardial lesions and passive hepatic congestion, which were identical to those previously documented in cases of bovine dilated cardiomyopathy. Skeletal muscles represented a vacuolar myopathic lesion characterized by the presence of distinctive vacuoles in atrophic myofibers, which occurred singly in the central zone of each myofiber on transverse sections and showed an irregularly elongated appearance on longitudinal sections. Such vacuoles frequently contained a small amount of weakly eosinophilic, granular, debris-like, or amorphous substances, displaying a feature of autophagic vacuoles. These vacuolated myofibers were more prominently observed in some muscle fascicles of the longissimus thoracis muscle than other muscles examined. The vacuolar myopathic lesion recognized in this cow was similar to some degree to that of autophagic vacuolar myopathy in man. Further studies are necessary to clarify a causal relationship between this vacuolar myopathic lesion and the d...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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In this study, we investigated the role of miR-223-3p in experimental autoimmune myocarditis (EAM). We found that miR-223-3p expression was significantly lower in EAM mice than that in normal mice. miR-223-3p inhibited NLRP3 inflammasome expression, promoting the polarization of dendritic cells (DCs) towards a tolerogenic DC phenotype. miR-223-3p effectively induced regulatory T cell (Treg) generation by inhibiting the function of antigen-presenting DCs. Transfer of miR-223-3p-overexpressing DCs protected mice against the development of EAM. Our findings suggest that miR-223-3p is involved in the induction of the tolerogen...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN01696A, PaperJing Huang, Anuradha Ramoji, Shuxia Guo, Thomas Bocklitz, Val érie Jahns, Jan Möller, Michael Kiehntopf, Michel Noutsias, Jürgen Popp, Ute Neugebauer Dilated cardiomyopathy (DCM) is a leading cardiomyopathy entity and is the leading reason for heart transplantation. Due to high etiologic and genetic heterogeneity of the pathologies, different therapeutic treatment strategies... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research
This study provides a new strategy for research on DCM or other diseases. Furthermore, lncRNA-miRNA pairs may be regarded as candidate diagnostic biomarkers or potential therapeutic targets of DCM.
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
We investigated a dilated cardiomyopathy (DCM) mutation (F764L) in human β-cardiac myosin by determining its motor properties in the presence and absence of the heart failure drug omecamtive mecarbil (OM). The mutation is located in the converter domain, a key region of communication between the catalytic motor and lever arm in myosins, and is nearby but not directly in the OM-binding site. We expressed and purified human β-cardiac myosin subfragment 1 (M2β-S1) containing the F764L mutation, and compared it to WT with in vitro motility as well as steady-state and transient kinetics measurements. In the absen...
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Enzymology Source Type: research
RASopathies are a group of rare genetic diseases caused by germline mutations in genes involved in the RAS–mitogen-activated protein kinase (RAS-MAPK) pathway. Whole-exome sequencing (WES) is a powerful approach for identifying new variants in coding and noncoding DNA sequences, including miRNAs. miRNAs are fine-tuning negative regulators of gene expression. The presence of variants in miRNAs could lead to malfunctions of regulation, resulting in diseases. Here, we identified 41 variants in mature miRNAs through WES analysis in five patients with previous clinical diagnosis of RASopathies syndromes. The pathways, bio...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
In this study, we investigated the long noncoding RNA (lncRNA) and messenger RNA (mRNA) expressions in the plasma of patients with DCM and ICM and analyzed the different lncRNA profile between the two groups. The microarray analysis identified 3,222 and 1,911 significantly differentially expressed lncRNAs and mRNAs between DCM and ICM group. The most enriched upregulated functional terms included positive regulation of I-kappaB kinase/nuclear factor-kappaB signaling and regulation of cellular localization, while the top 10 downregulated genes mainly consisted of acid secretion and myosin heavy chain binding. Furthermore, t...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
This study compared the myocardial deformation patterns of ICM and NICM using 2-D speckle tracking echocardiography (2-D STE) and sought to find parameters valuable in the diagnosis and management of dilated cardiomyopathy. The study population comprised 84 consecutive patients with LV end-diastolic dimension>55 mm and ejection fraction (EF)
Source: Ultrasound in Medicine and Biology - Category: Radiology Authors: Tags: Original Contribution Source Type: research
We report a case of an 8-year-old girl who presented with syncopal attacks and a history of viral illness a month ago. On examination, she was conscious, oriented but had a heart rate of 42/min which was unresponsive to atropine. She was started on dobutamine and isoproterenol. Electrocardiography and echocardiography revealed complete heart block with moderate tricuspid regurgitation, dilated cardiomyopathy and low ejection fraction. Patient was planned for urgent permanent pacemaker insertion. General anaesthesia was administered with endotracheal tube and controlled ventilation using fentanyl, ketamine and pancuronium. ...
Source: Indian Journal of Anaesthesia - Category: Anesthesiology Authors: Source Type: research
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Source: Global Health Action - Category: International Medicine & Public Health Tags: Glob Health Action Source Type: research
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Source: European Journal of Internal Medicine - Category: Internal Medicine Authors: Tags: Eur J Intern Med Source Type: research
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