Thapsigargin Induces Apoptosis When Autophagy is Inhibited in HepG2 Cells and Both Processes are Regulated by ROS-dependent pathway

Publication date: Available online 9 December 2015 Source:Environmental Toxicology and Pharmacology Author(s): Congcong Wang, Tao Li, Shusheng Tang, Dongxu Zhao, Chaoming Zhang, Shen Zhang, Sijun Deng, Yan Zhou, Xilong Xiao Thapsigargin (TG), is widely used to induce endoplasmic reticular stress. Treated with TG for a long time, cells suffer the Unfolded Protein Response (UPR) to elude apoptosis, but may activate autophagy. However, the switch between autophagy and apoptosis is unclear. To clarify the key signal for selection of these two protective responses, we studied the correlation of autophagy and apoptosis in HepG2 cells exposed to TG with time. TG induced apoptosis in HepG2 cells was evidenced by typical cell morphological changes and the activation of caspase-12, caspase-9 and caspase-3. Meanwhile, cytochrome c was released following with the dissipation of mitochondrial membrane potential (MMP), and the ratio of Bax/Bcl-2 was increased. TG-induced autophagy was confirmed by the accumulation of MDC, GFP-LC3 staining autophagic vacuoles, and the improved expression of LC3 II and Beclin-1. Additionally, inhibited autophagy via chloroquine (CQ) markedly enhanced the apoptosis induced by TG, which was linked to the Bcl-2 family. Furthermore, TG induced the generation of reactive oxygen species (ROS), and the ROS scavenger effectively suppressed TG-induced apoptosis and autophagy. All these results proved that restraint of autophagy may enhance TG-in...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research