Two fungal endophytes reduce the severity of pitch canker disease in Pinus radiata seedlings

In this study, 154 endophyte isolates were selected from a collection of 546 fungi tested in a preliminary confrontation assay. These isolates were then tested against F. circinatum in an in vitro antagonism experiment. Four different types of indicators (length of the central axis of the colony of the pathogen, the shape coefficient, percentage inhibition of radial growth and percentage inhibition zone) were used to detect and quantify the antagonistic activity directed towards the pathogen by the endophytes. The six isolates that showed the most promising results were inoculated in the field, together with the pathogen, into seedlings of Pinus radiata, P. sylvestris, P. pinaster, P. nigra and P. pinea, to test whether they could reduce the damage caused by F. circinatum. In total, 138 endophytes displayed antagonistic activity towards F. circinatum in the dual cultures of the in vitro experiment. In the field test, the endophytes Chaetomium aureum and Alternaria sp. reduced the area under disease progress curve (AUDPC) for the P. radiata seedlings, indicating that they may therefore be suitable for use as biological control agents (BCAs) of the disease. Graphical abstract
Source: Biological Control - Category: Biology Source Type: research