Quantitative PCR Analysis and Protein Distribution of Drug Transporter Genes in the Rat Cochlea.

In this study, we examined the levels of endogenous membrane transporters in rat cochlea by targeted PCR array analysis of 84 transporter genes, followed by validation and localization in tissues by immunohistochemistry. Our studies indicate that several members of the SLC, VDAC and ABC membrane transporter families show high levels of expression, both at the RNA and protein levels in the rat cochlea. Identification and characterization of these membrane transporters in the inner ear have clinical implications for both therapeutic and cytotoxic mechanisms that may aid in the preservation of auditory function.
PMID: 26626361 [PubMed - as supplied by publisher]

Source: Hearing Research - November 25, 2015 Category: Audiology Authors: Manohar S, Jamesdaniel S, Ding D, Salvi R, Seigel GM, Roth JA Tags: Hear Res Source Type: research