Monoclonal antibodies against G protein of spring viremia of carp virus.

Monoclonal antibodies against G protein of spring viremia of carp virus. Monoclon Antib Immunodiagn Immunother. 2014 Oct;33(5):340-3 Authors: Luo P, Ruan X, Zhang Q, Li Z, Wang M, Liu X Abstract Part of the G protein (3094-4170 bp) of spring viremia of carp virus (SVCV) was expressed in Escherichia coli and purified by dialysis in our study. Two clones of monoclonal antibodies (MAbs 1H11 and 4B8) against G protein were generated by fusion of mouse myeloma cell line SP2/0 and spleen lymphocytes from part of G protein (3094-4170 bp) immunized mice. The results of ELISA (enzyme-linked immunosorbent assay), IFA (indirect immunofluorescent assay), and Western blot assay further demonstrated the characterizations of the two MAbs. Both 1H11 and 4B8 were specific to SVCV G protein. Ten pairs of synthesized overlapping peptides were used to identify the epitope of the MAbs. The MAbs are useful in the development of SVCV diagnostic methods. PMID: 25358003 [PubMed - indexed for MEDLINE]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

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Publication date: Available online 26 September 2019Source: Protein Expression and PurificationAuthor(s): Yunpeng Shen, Masayo Morishita, Eric di LuccioAbstractThe nuclear receptor-binding SET Domain (NSD) family consists of NSD1, NSD2/MMSET/WHSC1, and NSD3/WHSC1L1 histone methyltransferases that are crucial for chromatin remodeling. NSDs are implicated in developmental disorders such as Wolf-Hirschhorn and Sotos syndromes as well as various cancers, including t(4; 14)(p16; q32) myeloma, an incurable cancer in plasma cells. NSDs have been the target of intensive study to understand their biological functions more fully and...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 12 July 2019Source: Protein Expression and PurificationAuthor(s): Patrick J. McDevitt, Jessica L. Schneck, Elsie Diaz, Wangfang Hou, Michael J. Huddleston, Rosalie E. Matico, Patricia M. McCormick, Robert B. KirkpatrickAbstractWolf-Hirschhorn Syndrome Candidate 1 (WHSC1; also known as NSD2) is a SET domain-containing histone lysine methyltransferase. A chromosomal translocation occurs in 15–20% of multiple myeloma patients and is associated with increased production of WHSC1 and poor clinical prognosis. To define the substrate requirements of NSD2, we established a platform for the ...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Acta Haematol
Source: Acta Haematologica - Category: Hematology Source Type: research
PMID: 31185477 [PubMed - as supplied by publisher]
Source: Acta Haematologica - Category: Hematology Authors: Tags: Acta Haematol Source Type: research
Conclusion and Future Perspectives This review illustrates our current knowledge of USP7, including its source and characterization, structure, binding partners and substrates in various biological processes. Besides, how USP7 regulates various aspects of a cell under both normal and pathological states are elaborated in detail. As the processes of ubiquitination and deubiquitination are extremely dynamic and context-specific, a series of studies have linked USP7 to different cancers. The biology, particularly the immune oncology mechanisms, reveal that USP7 inhibitors would be useful drugs, thus it is vital to develop hi...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
This study was supported by the Swiss National Science Foundation No. 31-124861 to GS. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Supplementary Material The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fphar.2019.00414/full#supplementary-material References Chava, K. R., Tauseef, M., Sharma, T., and Mehta, D. (2012). Cyclic AMP response element-binding protein prevents endothelial permeability...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Conclusions In conclusion, articles in this Research Topic made a very significant contribution to our understanding of the role played by environmental factors, dysbiotic conditions, and infections in triggering diseases. Since this is a rapidly expanding area of research, many other factors contributing to the onset of these diseases are not covered here. We are confident, however, that further studies will expand the list as well as bring a better understanding of mechanisms involved in the onset of autoimmune and autoinflammatory diseases. Author Contributions All authors listed have made a substantial, direct and i...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Mariana Girão1,2, Inês Ribeiro1, Tiago Ribeiro1, Isabel C. Azevedo1, Filipe Pereira1, Ralph Urbatzka1, Pedro N. Leão1 and Maria F. Carvalho1* 1Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal 2Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal Nature is the major reservoir of biologically active molecules. The urgent need of finding novel molecules for pharmaceutical application is prompting the research of underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria asso...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Preparation of a monoclonal antibody against the carcinoembryonic antigen, glypican‑3. Mol Med Rep. 2019 Mar 13;: Authors: Zhang Y, Qiu D, Li R, Liu Y, Shi S, Wang Y Abstract The carcinoembryonic antigen, glypican‑3 (GPC3), is a putative therapeutic target and diagnostic marker of hepatoma. In the present study, a monoclonal antibody (mAb) specifically against GPC3 was obtained via cloning the sequence of GPC3 via polymerase chain reaction and inserting it into a pET16b vector prior to transfection into Escherichia coli (E. coli) BL21. BALB/c mice were immunized with 20 µg...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Conclusion: In our experience using the PCR- Biofire assay we show that this approach is feasible and can be used safely to tailor therapy in immunocompromised patients. In all cases, E.Coli was found, most frequently STEC (Shiga-like toxin producing E. coli). Using this approach enable us to identify new pathogens causing diarrhea in immunocompromised patients, extremely quickly. These preliminary results reveal the need for more clinical experience using the Biofire FilmArray as a diagnostic test for patients with MM and other haematological malignancies, who present acutely with severe diarrhea.DisclosuresTadmor: NOVART...
Source: Blood - Category: Hematology Authors: Tags: 203. Lymphocytes, Lymphocyte Activation, and Immunodeficiency, including HIV and Other Infections Source Type: research
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