Generation and characterization of a monoclonal antibody against prM protein of West Nile virus.

In this study we generated and characterized a monoclonal antibody (MAb) against the WNV prM protein. Western blot analysis showed that the MAb reacted with WNV prM specifically. Immunohistochemistry assays demonstrated that the MAb recognized native prM protein in transfected BHK-21 cells. Preliminary studies were performed to identify the epitope recognized by the MAb using a set of synthesized overlapping peptides spanning the whole length of the prM protein. The MAb reported here may provide a valuable tool for the further exploration of the biological properties and functions of the prM protein and may also be developed for potential clinical applications. PMID: 25514166 [PubMed - in process]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

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idt-Chanasit Daniel Cadar Renke Lühken The ecology of West Nile virus (WNV) in the Danube Delta Biosphere Reserve (Romania) was investigated by combining studies on the virus genetics, phylogeography, xenosurveillance and host-feeding patterns of mosquitoes. Between 2014 and 2016, 655,667 unfed and 3842 engorged mosquito females were collected from four sampling sites. Blood-fed mosquitoes were negative for WNV-RNA, but two pools of unfed Culex pipiens s.l./torrentium collected in 2014 were tested positive. Our results suggest that Romania experienced at least two separate WNV lineage 2 introductions: from Afr...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
(European Centre for Disease Prevention and Control (ECDC)) Nearly one in three foodborne outbreaks in the EU in 2018 were caused by Salmonella. This is one of the main findings of the annual report on trends and sources of zoonoses published by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC). West Nile virus and STEC infections were reported at unusually high levels.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
CONCLUSION: WNV NAT's utility is between June-December; however, abandoning testing outside of this time may increase risk. While in-process testing identified approximately one ID-NAT-only detectable (antibody-negative) donation per year, reducing the geographic triggered area decreased its effectiveness. PMID: 31829449 [PubMed - as supplied by publisher]
Source: Transfusion - Category: Hematology Authors: Tags: Transfusion Source Type: research
Conclusion: Mosquitoes and midges have got a crucial role in the transmission of arboviruses. The risk of occurrence for the investigated arboviruses will continue depending upon many factors including the presence of these viruses in Turkey and its neighboring countries, uncontrolled livestock movements, global warming and climate changes. PMID: 31803784 [PubMed]
Source: Iranian Journal of Arthropod-Borne Diseases - Category: Infectious Diseases Tags: J Arthropod Borne Dis Source Type: research
No abstract available
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Errata Source Type: research
Conclusions: Two large areas were found as the ecological niches of larvae of these species. It is recommended that additional investigations be carried out on infection in adult female Cx. pipiens and its hosts in these areas. PMID: 31745415 [PubMed]
Source: Oman Medical Journal - Category: Middle East Health Tags: Oman Med J Source Type: research
West Nile virus (WNV) is a neurotropic flavivirus and the leading cause of mosquito-borne diseases in North America. In approximately 20% of cases, infection leads to a self-limited febrile illness and, in less than 1%, to a neuroinvasive disease often manifesting as meningoencephalitis with or without acute flaccid paralysis.1 Herein, we report the first case of WNV-associated CNS vasculitis and intracranial hemorrhage, thus expanding the spectrum of WNV infection.
Source: Neurology Neuroimmunology and Neuroinflammation - Category: Neurology Authors: Tags: Vasculitis, Encephalitis, Viral infections, Intracerebral hemorrhage, Subarachnoid hemorrhage Clinical/Scientific Notes Source Type: research
Publication date: 19 November 2019Source: Cell Reports, Volume 29, Issue 8Author(s): Chulwoo Kim, Rohit R. Jadhav, Claire E. Gustafson, Megan J. Smithey, Alec J. Hirsch, Jennifer L. Uhrlaub, William H. Hildebrand, Janko Nikolich-Žugich, Cornelia M. Weyand, Jörg J. GoronzySummaryGeneration of protective immunity to infections and vaccinations declines with age. Studies in healthy individuals have implicated reduced miR-181a expression in T cells as contributing to this defect. To understand the impact of miR-181a expression on antiviral responses, we examined LCMV infection in mice with miR-181ab1-deficient T&nbs...
Source: Cell Reports - Category: Cytology Source Type: research
Authors: Whyler NC, Teng JC, Brewster DJ, Chin R, Cox I, Druce J, Prince HM, Sheffield DA, Teh E, Sarode V PMID: 31736076 [PubMed - as supplied by publisher]
Source: Medical Journal of Australia - Category: General Medicine Tags: Med J Aust Source Type: research
We present evidence that these observations are only partly explained by differences in nucleotide composition of the complete viral codon regions. We consider the combination of nucleotide composition, amino acid composition, and codon usage preference suitable to provide information on possible evolutionary similarities between distant virus species that cannot be investigated by phylogeny.
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research
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