Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistant Staphylococcus aureus.

Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistant Staphylococcus aureus. Monoclon Antib Immunodiagn Immunother. 2015 Aug;34(4):257-62 Authors: Senna JP, Teixeira Mda G, Santiago Mde A, Batoréu NM, Valadares N, Galler R Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium that causes serious infections worldwide. This pathogen is resistant to all beta lactam antibiotics due the presence of PBP2a, a transpeptidase enzyme that presents very low beta-lactam affinity. Here we report the generation and characterization of mouse monoclonal antibodies to PBP2a of MRSA strains. Two clones were obtained and characterized by immunoassays (ELISA, avidity index determination, and immunoblotting), isotyping, association/dissociation rate constants by surface plasmon resonance (SPR), and flow cytometry. Clone 38, which showed the best avidity and affinity, bound to PBP2a located on the bacterial surface by flow cytometry. Further studies are warranted in order to evaluate if these antibodies may help inhibit bacterial growth and be used to treat infections by MRSA. PMID: 26301929 [PubMed - in process]
Source: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy - Category: Microbiology Tags: Monoclon Antib Immunodiagn Immunother Source Type: research

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Staphylococcus aureus is one of the major causes of nosocomial infections. This organism produces powerful toxins and cause superficial lesions, systemic infections, and several toxemic syndromes. A total of 109 S. aureus strains isolated from a variety of infections like ocular diseases, wound infection, and sputum were included in the study. Minimum inhibitory concentration (MIC) was determined against 8 antimicrobials. PCR determined the presence of 16S rRNA, nuc, mecA, czrC, qacA/B, pvl, and toxin genes in S. aureus isolates. Pulse-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), SCCmec, spa-, and ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 20 November 2019Source: Journal of Analytical and Applied PyrolysisAuthor(s): M. Ibrahimi, W. Korichi, S. Loqman, M. Hafidi, Y. Ouhdouch, L. LemeeAbstractThermochemolysis-GC-MS was used to characterize Methicillin resistant Staphylococcus aureus (MRSA) and a predatory actinobacteria isolated from Moroccan marine water through their membrane fatty acids released as methyl esters (FAMEs).FAMEs from MRSA were dominated by branched iso and anteiso C15, straight C18 and C20 whereas the predatory actinobacteria was dominated by branched C14, iso and anteiso C15, and straight C16. The iso to ant...
Source: Journal of Analytical and Applied Pyrolysis - Category: Chemistry Source Type: research
Publication date: Available online 6 November 2019Source: Clinics and Research in Hepatology and GastroenterologyAuthor(s): Manon Allaire, Jean-François Cadranel, Thi Thu Nga Nguyen, Armand Garioud, Honore Zougmore, Ratmony Heng, Claire Perignon, Isabelle Ollivier-Hourmand, Thông DaoSummaryPatients with cirrhosis are prone to develop bacterial infections, which consist in one of the major precursors of Acute-on-Chronic Liver Failure (ACLF) and are responsible for a high mortality rate. In recent years, the management of bacterial infections in patients with cirrhosis has become increasingly complicated due to ...
Source: Clinics and Research in Hepatology and Gastroenterology - Category: Gastroenterology Source Type: research
Authors: Yu H, Wang Y, Wang X, Guo J, Wang H, Zhang H, Du F Abstract Bacterial resistance to antimicrobial agents, including multidrug resistance, is an increasing problem in the treatment of infectious diseases. The development of resistance-modifying agents represents a potential strategy to alleviate the spread of bacterial resistance to antibiotics. A checkerboard microdilution assay was used to determine the synergy of jatrorrhizine and the antibiotic, norfloxacin (NFX). A bacterial ethidium bromide efflux assay, reverse transcription semi-quantitative polymerase chain reaction analysis and molecular docking s...
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
Publication date: Available online 15 October 2019Source: American Journal of Infection ControlAuthor(s): Hiroki Kitagawa, Minako Mori, Seiya Kashiyama, Yayoi Sasabe, Kiyoko Ukon, Naomi Shimokawa, Nobuaki Shime, Hiroki OhgeBackgroundThe hospital environment is an important source of multidrug-resistant organisms such as methicillin-resistant Staphylococcus aureus (MRSA). Here, we evaluated the efficacy of pulsed xenon ultraviolet (PX-UV) disinfection in addition to manual cleaning in a Japanese hospital.MethodsEnvironmental samples were collected from inpatient rooms that had been occupied for at least 48 hours by patients...
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Staphylococcus aureus, particularly methicillin-resistant S. aureus (MRSA), is an important human pathogen that exhibit enhanced virulence and resistance to different antibiotics. It causes a wide range of infections from mild to life-threatening conditions, both in the hospital (healthcare-associated-infections/HAIs) and in the community (community-associated-infections/CAIs), resulting in increased costs for the healthcare system [1,2]. Although there has been a recent decrease in HAIs (but not among CA invasive infections), caused by MRSA, in EEUU [3] and in some European countries [4], the attributable mortality due to...
Source: Journal of Infection - Category: Infectious Diseases Authors: Source Type: research
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
In conclusion, all these findings presented in this work convincingly suggested that polymer 2a may be a promising therapeutic alternative for treating MRSA-induced infections, especially bloodstream infection. PMID: 31548363 [PubMed - as supplied by publisher]
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research
Abstract One of the largest concerns in public health is the continual emergence of multidrug-resistant bacterial pathogens. The resistance of bacterial pathogens to specific drugs presents a significant problem because it severely limits treatment options. Staphylococcus aureus is a particularly problematic pathogen that is prevalent in human and animal populations. Data on this bacterium have shown that S. aureus is capable of invading different types of host cells, suggesting that multiple mechanisms are behind its ability to thwart a host immune system and evade the toxicity of some antibiotics. S. aureus prod...
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research
ConclusionThis study describes the main features of the draft genome of S. agnetis 3682, a strain producing the first bacteriocin (agneticin 3682) reported in this species. A second gene cluster encoding a sactipeptide was also found in the bacterial chromosome. Agneticin 3682 shows a new potential application against clinical MRSA isolates.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
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