microRNA-33A expression is reduced in cerebral cortex in a rat model of ischemic tolerance.

This study examined the microRNA—33a (miR—33a) expression levels in cerebral cortex in rat model of cerebral ischemic tolerance (IT), with 3—nitropropionic acid (3—NPA) preconditioning. Rat model of cerebral IT was established as follows: 14 male Sprague—Dawley rats were randomly divided into two groups, the 3—NPA treated group and the control group. Intraperitoneal injection of 3—NPA or normal saline was performed in 3—NPA treated group and control group, respectively. Middle cerebral artery occlusion (MCAO) was performed in all the rats 3 days after injection and the infarct volume was measured on postoperative day—2. To study miR—33a expression levels in this model, male Sprague—Dawley rats (n = 31) were randomly divided into 4 groups, 3—NPA treatment group (n = 8), 3—NPA treatment + MCAO group (n = 9), MCAO group (n = 9), and control group (n = 5). Surviving rats were sacrificed 4 days after injection and cerebral cortex samples were obtained for total RNA isolation. MiR—33a expression levels were determined by real—time quantitative PCR (qRT—PCR). Infarct volume in 3—NPA treated group decreased significantly in comparison to the control group (P < 0.05). Expression analysis by qRT—PCR revealed that miR—33a expression levels in 3—NPA treated group were significantly hi...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research