Radioprotective effect of chicory seeds against genotoxicity induced by ionizing radiation in human normal lymphocytes.

Radioprotective effect of chicory seeds against genotoxicity induced by ionizing radiation in human normal lymphocytes. Cell Mol Biol (Noisy-le-grand). 2015;61(4):46-50 Authors: Hosseinimehr SJ, Ghaffari-Rad V, Rostamnezhad M, Ghasemi A, Allahverdi Pourfallah T, Shahani S Abstract The search for less—toxic radioprotective agents has led to a growing trend towards natural products. Protective effect of the methanolic extract of chicory seeds (MCS) was investigated against genotoxicity induced by ionizing radiation in human lymphocytes. Human peripheral blood samples were collected and incubated with MCS at different concentrations (10, 50, 100, and 200 μg/mL) for two hours. The whole blood samples were exposed in vitro to X—ray at dose 2.5 Gy. Then, the lymphocytes were cultured with mitogenic stimulation to determine the micronucleus in cytokinesis blocked binucleated cell. The methanolic extract at all doses significantly reduced the frequency of micronuclei in binucleated lymphocytes, as compared with similarly irradiated lymphocytes without any extract treatment. The maximum protection was observed at 200 μg/mL of MCS, it completely protected genotoxicity induced by ionizing radiation in human lymphocytes. The extract exhibited a concentration—dependent radical scavenging activity on 1,1—diphenyl—2—picryl hydrazyl free radicals. HPLC analysis of MCS sho...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research