Progesterone promotes cell migration, invasion and cofilin activation in human astrocytoma cells

Publication date: Available online 27 November 2015 Source:Steroids Author(s): Ana Gabriela Piña-Medina, Valeria Hansberg-Pastor, Aliesha González-Arenas, Marco Cerbón, Ignacio Camacho-Arroyo Astrocytomas are the most common and aggressive primary brain tumors in humans. Invasiveness of these tumors has been attributed in part to deregulation of cell motility-dependent cytoskeletal dynamics that involves actin-binding proteins such as cofilin. Progesterone (P4) has been found to induce migration and invasion of cells derived from breast cancer and endothelium. However, the role of P4 in migration and invasion of astrocytoma cells as well as its effects on astrocytomas cytoskeleton remodeling is not known. In this work we evaluated these aspects in D54 and U251 cells derived from human astrocytomas from the highest degree of malignancy (grade IV, glioblastoma). Our results showed that in scratch-wound assays P4 increased the number of D54 and U251 cells migrating from 3 to 48 hours. Both RU486, a P4 receptor (PR) antagonist, and an oligonucleotide antisense against PR significantly blocked P4 effects. Transwell assays showed that P4 significantly increased the number of invasive cells at 24 hours. As in the case of migration, this effect was blocked by RU486. Finally, by western blotting, an increase in the cofilin/p-cofilin ratio at 15 and 30 minutes and a decrease at 30 and 60 minutes in U251 and D54 cells, respectively, was observed after P4, P4 + RU486 and...
Source: Steroids - Category: Drugs & Pharmacology Source Type: research