Detection of Circulating in Patients with Papillary Thyroid Carcinoma
BRAFV600E is a common mutation in papillary thyroid carcinoma (PTC) correlated with aggressive features. Our objective was to assess the feasibility and accuracy of a novel RNA-based blood assay to identify individuals with a high-risk tumor mutation in patients with PTC. Patients with benign or malignant thyroid disorders were included between September 2013 and July 2014 before either thyroidectomy (n = 62) or treatment of recurrent or metastatic PTC (n = 8). RNA was isolated from peripheral blood lymphocytes and reverse transcribed and followed by two rounds of nested PCR amplification with a restriction digest specific for wild-type BRAF.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Carrie C. Lubitz, Sareh Parangi, Tammy M. Holm, M. Jordana Bernasconi, Aislyn P. Schalck, Hyunsuk Suh, Konstantinos P. Economopoulos, Viswanath Gunda, Samuel E. Donovan, Peter M. Sadow, Lori J. Wirth, Ryan J. Sullivan, David J. Panka Tags: Regular Article Source Type: research