High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation

Serum from bar-coded tubes, and then internal standard, are pipetted to 96-well plates with an 8-channel automated liquid handler (ALH). The first precipitation reagent (methanol:ZnSO4) is added and mixed with the 8-channel ALH. A second protein precipitating agent, 1 % formic acid in acetonitrile, is added and mixed with a 96-channel ALH. After a 4-min delay for larger precipitates to settle to the bottom of the plate, the upper 36 % of the precipitate/supernatant mix is transferred with the 96-channel ALH to a Sigma Hybrid SPE® plate and vacuumed through for removal of phospholipids and precipitated proteins. The filtrate is collected in a second 96-well plate (collection plate) which is foil-sealed, placed in the autosampler (ALS), and injected into a multiplexed LC-MS/MS system running AB Sciex Cliquid® and MPX® software. Two Shimadzu LC stacks, with multiplex timing controlled by MPX® software, inject alternately to one AB Sciex API-5000 MS/MS using positive atmospheric pressure chemical ionization (APCI) and a 1.87 min water/acetonitrile LC gradient with a 2.1 × 20 mm, 2.7 μm, C18 fused core particle column (Sigma Ascentis Express). LC-MS/MS through put is ~44 samples/h/LC-MS/MS system with dual-LC channel multiplexing. Plate maps are transferred electronically from the ALH and reformatted into LC-MS/MS sample table format using the Data Innovations LLC (DI) Instrument Manager middleware application. Before collection plates are lo...
Source: Springer protocols feed by Imaging/Radiology - Category: Radiology Source Type: news

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AbstractLayered double hydroxides (LDHs) are 2D ionic lamellar nanomaterials, which attract increasing interest for applications in different fields. This paper shows that their morphology is strongly affected by the microstructure of the substrate on which they grow. Zn –Al LDHs were grown in the same conditions on Al substrates with different microstructural features obtained from cold-rolled sheets by annealing at 265 °C for increasing soaking time up to 1 h. LDH morphology was then investigated through scanning electron microscopy observations. The homogene ous distribution of petals on the substrat...
Source: European Journal of Applied Physiology - Category: Physiology Source Type: research
ABSTRACT This research evaluated the effect of different delignification pretreatments (enzymatic and organosolv), on the crystallinity and enzymatic hydrolysis of harvested sugar cane residues. The Crystallinity Index (CrI), the Relative Number of Intensity (Ir), the degree of cellulose mercerization (IIC-%), and the Global Index of Saccharification (GIS) were used as measurement parameters for six different substrates obtained from sugar cane residues (tops and leaves) by different processes. In this characterization, the spectroscopic ty Techniques of Fourier Transform infrared spectroscopy (FTIR), X-ray diffraction and...
Source: Brazilian Journal of Chemical Engineering - Category: Chemistry Source Type: research
CONCLUSIONS: Accuracy of CT scan for identification of pT3/4, pN+, or extramural venous invasion was insufficient to allow for proper identification of patients at high risk for local recurrence and/or in whom to consider alternative treatment strategies. Locoregional overstaging and understaging resulted in inappropriate treatment strategies in
Source: Diseases of the Colon and Rectum - Category: Gastroenterology Tags: Original Contributions: Colorectal Cancer Source Type: research
Apples are among the most consumed fruits world-wide. They represent a source of direct human exposure to bacterial communities, which is less studied. We analyzed the apple microbiome to detect differences between tissues and the impact of organic and conventional management by a combined approach of 16S rRNA gene amplicon analysis and qPCR, and visualization using fluorescence in situ hybridization and confocal laser scanning microscopy (FISH-CLSM). Each apple fruit harbors different tissues (stem, peel, fruit pulp, seeds and calyx), which were colonized by distinct bacterial communities. Interestingly, fruit pulp and ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The Department of Justice is reportedly investigating claims that Johnson and Johnson officials lied about cancer risks tied to its popular baby powder.
Source: PharmaManufacturing.com - Category: Pharmaceuticals Source Type: news
The visibility of screen-detected cancers is generally equal to or higher on...Read more on AuntMinnie.comRelated Reading: Can DBT replace FFDM? SBI: DBT users aren't implementing synthesized 2D Synthesized 2D mammography works just as well as FFDM Breast tissue seems less dense on DBT scans Synthesized 2D mammo + DBT works for breast screening
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
Authors: Khairnar H, Ingle M, Chauhan S, Pipalia N, Sawant P, Pandey V, Shukla A Abstract Introduction: Computed Tomography of abdomen frequently shows bowel wall thickening with different grades and characteristic of thickening. The correlation of bowel wall thickening (BWT) with endoscopic findings is not well described in Indian population. Therefore we did this study to determine the correlation of BWT with endoscopic findings. Methods: Its Prospective single center study with 85 Consecutive patients with age group more than 12 years with CT scan abdomen showing bowel wall thickening were included in the st...
Source: Journal of the Association of Physicians of India - Category: General Medicine Tags: J Assoc Physicians India Source Type: research
Centerline Biomedical, a company based in Cleveland, Ohio, landed FDA clearance for its Intra-Operative Positioning System (IOPS). The product provides physicians with a radiation-free way to navigate through vasculature during minimally invasive pro...
Source: Medgadget - Category: Medical Devices Authors: Tags: Cardiac Surgery Cardiology Radiology Vascular Surgery Source Type: blogs
LOS ANGELES (AP) — Scientists are closing in on a long-sought goal — a blood test to screen people for possible signs of Alzheimer’s disease and other forms of dementia. On Monday at the Alzheimer’s Association International Conference, half a dozen research groups gave new results on various experimental tests, including one that seems 88% accurate at indicating Alzheimer’s risk. Doctors are hoping for something to use during routine exams, where most dementia symptoms are evaluated, to gauge who needs more extensive testing. Current tools such as brain scans and spinal fluid tests are too ex...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - Category: Consumer Health News Authors: Tags: Health News Alzheimer's Disease Dementia Source Type: news
Established characterization methods for repetitive pulse trains tend to fail in the presence of degraded coherence properties. Partial coherence is present in several pulse generation schemes but is not yet properly addressed in many pulse characterization techniques. Full coherence is a common ...
Source: Journal of the Optical Society of America B - Category: Physics Authors: Source Type: research
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