High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
Measurement of methylmalonic acid (MMA) plays an important role in the diagnosis of vitamin B12 deficiency. Vitamin B12 is an essential cofactor for the enzymatic carbon rearrangement of methylmalonyl-CoA (MMA-CoA) to succinyl-CoA (SA-CoA), and the lack of vitamin B12 leads to elevated concentrations of MMA. Presence of succinic acid (SA) complicates the analysis because mass spectra of MMA and SA are indistinguishable, when analyzed in negative ion mode and the peaks are difficult to resolve chromatographically. We developed a method for the selective analysis of MMA that exploits the significant difference in fragmentation patterns of di-butyl derivatives of the isomers MMA and SA in a tandem mass spectrometer when analyzed in positive ion mode. Tandem mass spectra of di-butyl derivatives of MMA and SA are very distinct; this allows selective analysis of MMA in the presence of SA. The instrumental analysis is performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in positive ion mode, which is, in combination with selective extraction of acidic compounds, is highly selective for organic acids with multiple carboxyl groups (dicarboxylic, tricarboxylic, etc.). In this method organic acids with a single carboxyl group are virtually undetectable in the mass spectrometer; the only organic acid, other than MMA, that is detected by this method is its isomer, SA. Quantitative measurement of MMA in this method is performed using a deconvolution algorithm, which ma...
VITAMIN B12 deficiency symptoms may appear if a person lacks the vital vitamin from their diet. It ’s important to recognise the symptoms, because left untreated, vitamin B12 deficiency can lead to serious health complications. One sign of a deficiency may appear on the tongue.
VITAMIN B12 deficiency symptoms can develop if a person lacks B12 from their diet. Being able to recognise the symptoms is important because the condition left untreated can lead to serious health complications. A headache can be one of the signs, but what specifically should you be looking out for?
VITAMIN B12 is an essential nutrient for the body. A lack of the vitamin can impair bodily functions, leading to serious health problems if left untreated. There are various symptoms to watch out for, including this unusual sign.
VITAMIN B12 deficiency is a common condition but could be serious if left untreated as vitamin B12 is an essential vitamin. If your face looks like this it could mean you are lacking the vitamin. Does your face look like this?
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VITAMIN B12 deficiency symptoms can occur if a person lacks the vitamin from their diet. Recognising the symptoms is very important because left untreated, serious, long-term health complications can occur. One sign to watch out for is a certain feeling in the chest.
Introduction: Abuse of nitrous oxide (N2O) has an unusually high lifetime prevalence in developed countries and represents a serious concern worldwide. Myeloneuropathy following the inhalant abuse is commonly attributed to the disturbance of vitamin B12 metabolism, with severe motor deficits are often noted. The present study aims to elucidate its underlying pathophysiology. Methods: Eighteen patients with N2O abuse or vitamin B12 deficiency were recruited. Comprehensive central and peripheral neuro-diagnostic tests were performed, including whole spine MRI, and thermal quantitative sensory testing (QST). Specifically, pa...
VITAMIN B12 deficiency symptoms can occur if a person lacks the vital vitamin. Left untreated, serious long-term health complications can occur, so it ’s important to spot the signs. One symptom of the condition to look out for may appear on the lips.
In conclusion the prevalence of B12 deficiency in KTR was estimated as 14% and was associated with reduced intake of B12 as well as higher adiposity, especially in women, and with the use of MMF. PMID: 31280734 [PubMed - as supplied by publisher]
VITAMIN B12 deficiency symptoms are important to recognise because left untreated, serious long-term health complications can occur. Certain feelings in the body can signal the condition, and there are three to watch out for.