Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae.

Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae. Am J Trop Med Hyg. 2015 Nov 23; Authors: Aurélien FF, Mediannikov O, Nappez C, Azza S, Raoult D, Drancourt M Abstract Relapsing fever borreliae, produced by ectoparasite-borne Borrelia species, cause mild to deadly bacteremia and miscarriage. In the perspective of developing inexpensive assays for the rapid detection of relapsing fever borreliae, we produced 12 monoclonal antibodies (MAbs) against Borrelia crocidurae and characterized the two exhibiting the highest titers. P3A10 MAb reacts with the 35.6-kDa flagellin B (flaB) of B. crocidurae while P6D9 MAb recognizes a 35.1-kDa variable-like protein (Vlp) in B. crocidurae and a 35.2-kDa Vlp in Borrelia duttonii. Indirect immunofluorescence assay incorporating relapsing fever and Lyme group borreliae and 11 blood-borne organisms responsible for fever in West Africa confirmed the reactivity of these two MAbs. Combining these two MAbs in indirect immunofluorescence assays detected relapsing fever borreliae including B. crocidurae in ticks and the blood of febrile Senegalese patients. Both antibodies could be incorporated into inexpensive and stable formats suited for the rapid point-of-care diagnosis of relapsing fever. These first-ever MAbs directed against African relapsing fever borreliae are available for the scientific community to promote research in this neglected field. PMID: 26598566 [PubMed - as supplied by publisher]
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research

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It’s finally getting warm here in New England, and most of us have plans to enjoy the beautiful weather. And that’s why the Centers for Disease Control and Prevention (CDC) recently released a report raising awareness about how to prevent the tickborne infections that typically occur during this time of the year. Lyme disease is probably the most well-known, and the one for which diagnosis and treatment are most controversial. What is Lyme disease? Several countries around the world, especially in the Northern Hemisphere, and all 50 states in the US have already reported cases of Lyme. The disease is caused by ...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Infectious diseases Source Type: blogs
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Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Aid Education Featured Gender Global Headlines Health Population Poverty & SDGs TerraViva United Nations Source Type: news
ConclusionsHigh rates of recurrent normal/abnormal karyotypes were observed in all groups with a majority of embryos presenting with recurrent abnormal morphology. Secondary RPL presented significantly more often with recurrent abnormal morphology compared to primary RPL. These findings offer prognostic information for the affected patient and might impact treatment choice.
Source: Journal of Assisted Reproduction and Genetics - Category: Reproduction Medicine Source Type: research
(Natural News) Research published in the European Journal of Microbiology and Immunology revealed yet another sweet benefit of stevia. Known for its natural sweetening properties, stevia was discovered to be a safer and even more effective alternative to conventional, toxic antibiotics in treating Lyme disease. The researchers from the University of New Haven used an alcohol extract...
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Clinical Significance of Human Papillomavirus Type 16 for Breast Cancer &Adenocarcinomas of Various Internal Organs and Alzheimer's Brain with Increased β-amyloid (1-42); Combined Use of Optimal Doses of Vitamin D3 and Taurine 3 times/day Has Significant Beneficial Effects of Anti-Cancer, Anti-Ischemic Heart, and Memory &Other Brain Problems By Significant Urinary Excretion of Viruses, Bacteria, and Toxic Metals &Substances. Acupunct Electrother Res. 2016;41(2):127-134 Authors: Omura Y Abstract Human Papillomavirus type 16 (HPV-16) has a significant role in various cancers and Alzheime...
Source: Acupuncture and Electro-Therapeutics Research - Category: Complementary Medicine Tags: Acupunct Electrother Res Source Type: research
In this study, possible role of another Oligopeptide ABC transporter, OppA4 in ospC expression was investigated by construction of an oppA4 deletion mutant and the complemented strain. Inactivation of oppA4 resulted an increased production of OspC, suggesting that OppA4 has a negative impact on ospC expression. Expression of ospC is controlled by Rrp2-RpoN-RpoS, the central pathway essential for mammal infection. We showed that increased ospC expression in the oppA4 mutant was due to an increased rpoS expression. We then further investigated how OppA4 negatively regulates this pathway. Two regulators, BosR and BadR, are kn...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Immune imbalance at the maternal-fetal interface plays a fundamental role in the pathogenesis of unexplained recurrent spontaneous abortion (URSA). Human amniotic epithelial cells (hAECs) possess pregnancy-friendly immunomodulatory effects. Here, we investigated how function of naive CD4+ T cells from URSA patients is affected by hAECs.
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American Journal of Reproductive Immunology, EarlyView.
Source: American Journal of Reproductive Immunology - Category: Allergy & Immunology Authors: Source Type: research
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Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
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