Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae.
Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae. Am J Trop Med Hyg. 2015 Nov 23; Authors: Aurélien FF, Mediannikov O, Nappez C, Azza S, Raoult D, Drancourt M Abstract Relapsing fever borreliae, produced by ectoparasite-borne Borrelia species, cause mild to deadly bacteremia and miscarriage. In the perspective of developing inexpensive assays for the rapid detection of relapsing fever borreliae, we produced 12 monoclonal antibodies (MAbs) against Borrelia crocidurae and characterized the two exhibiting the highest titers. P3A10 MAb reacts with the 35.6-kDa flagellin B (flaB) of B. crocidurae while P6D9 MAb recognizes a 35.1-kDa variable-like protein (Vlp) in B. crocidurae and a 35.2-kDa Vlp in Borrelia duttonii. Indirect immunofluorescence assay incorporating relapsing fever and Lyme group borreliae and 11 blood-borne organisms responsible for fever in West Africa confirmed the reactivity of these two MAbs. Combining these two MAbs in indirect immunofluorescence assays detected relapsing fever borreliae including B. crocidurae in ticks and the blood of febrile Senegalese patients. Both antibodies could be incorporated into inexpensive and stable formats suited for the rapid point-of-care diagnosis of relapsing fever. These first-ever MAbs directed against African relapsing fever borreliae are available for the scientific community to promote research in this neglected field. PMID: 26598566 [PubMed - as supplied by publisher]
We have known for more than half a century that the earth is heating up and scientists have long foretold the consequences. Yet in these same decades, the earth’s population has increased dramatically; the demand for convenience, quality of life, and consumer goods has expanded; energy consumption and CO2 emissions have skyrocketed; and we are living in the hottest climate on record. So why aren’t we doing more to stop this catastrophe? One way to answer this question is by contrasting our inertia with the successful response to another public health crisis: the AIDS epidemic. Act Up, the political movem...
American Journal of Reproductive Immunology, EarlyView.
This study presents metrics to help work out what Tommy's should measure and how Tommy's should report impact. The aim is to work out how each contributes to Tommy's desired outcomes, namely the reduction of stillbirth, preterm birth and miscarriage.
ConclusionsA deep investigation on the factors associated with adverse neonatal outcomes requires a risk stratification. In particular, great attention must be paid to the prevention and treatment of obesity.
In this study, we found that TXNIP deficiency induces accelerated senescent phenotypes of mouse embryonic fibroblast (MEF) cells under high glucose condition and that the induction of cellular ROS or AKT activation is critical for cellular senescence. Our results also revealed that TXNIP inhibits AKT activity by a direct interaction, which is upregulated by high glucose and H2O2 treatment. In addition, TXNIP knockout mice exhibited an increase in glucose uptake and aging-associated phenotypes including a decrease in energy metabolism and induction of cellular senescence and aging-associated gene expression. We propose that...
We describe these efforts as an example of individual patients’ evidence-based activism enabled by new media platforms and other technoscientific tools available to the public.
Publication date: Available online 14 September 2018Source: European Journal of Obstetrics &Gynecology and Reproductive BiologyAuthor(s): Pauliina Ellilä, Linda Laitinen, Miina Nurmi, Päivi Rautava, Mari Koivisto, Päivi Polo-KantolaAbstractObjectivesNausea and vomiting of pregnancy (NVP) is frequent, affecting up to 70–85% of pregnant women. However, severity of NVP especially in clinical practice is often uncertainly assessed and thus both under- and overdiagnosing is probable. Furthermore, risk factors for NVP, although recognized, are not well established. The aim of our study was to evaluate th...
ConclusionsPaternal HBV infection resulted in a lower frequency of clinical pregnancy after FET, a difference that was probably attributed to a detrimental effect of HBV on the ability of embryos to survive freezing and thawing.
ConclusionIn light of connecting etiological link between infertility and RM as reviewed in this study, interrogating the oocyte-specific genes with suspected roles in reproductive biology, in cases of unexplained RM, may open new possibilities in widening our understanding of RM pathophysiology.
Cellular Microbiology,Volume 0, Issue ja, -Not available-.