Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae.

Monoclonal Antibodies for the Diagnosis of Borrelia crocidurae. Am J Trop Med Hyg. 2015 Nov 23; Authors: Aurélien FF, Mediannikov O, Nappez C, Azza S, Raoult D, Drancourt M Abstract Relapsing fever borreliae, produced by ectoparasite-borne Borrelia species, cause mild to deadly bacteremia and miscarriage. In the perspective of developing inexpensive assays for the rapid detection of relapsing fever borreliae, we produced 12 monoclonal antibodies (MAbs) against Borrelia crocidurae and characterized the two exhibiting the highest titers. P3A10 MAb reacts with the 35.6-kDa flagellin B (flaB) of B. crocidurae while P6D9 MAb recognizes a 35.1-kDa variable-like protein (Vlp) in B. crocidurae and a 35.2-kDa Vlp in Borrelia duttonii. Indirect immunofluorescence assay incorporating relapsing fever and Lyme group borreliae and 11 blood-borne organisms responsible for fever in West Africa confirmed the reactivity of these two MAbs. Combining these two MAbs in indirect immunofluorescence assays detected relapsing fever borreliae including B. crocidurae in ticks and the blood of febrile Senegalese patients. Both antibodies could be incorporated into inexpensive and stable formats suited for the rapid point-of-care diagnosis of relapsing fever. These first-ever MAbs directed against African relapsing fever borreliae are available for the scientific community to promote research in this neglected field. PMID: 26598566 [PubMed - as supplied by publisher]
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research

Related Links:

Inspired Organics, LLC has issued a voluntary recall of Organic Almond Butter due to potential contamination of Listeria monocytogenes. Listeria monocytogenes is an organism, which can cause serious and sometimes fatal infections in young children, frail or elderly people, and others with weakened immune systems. Although healthy individuals may suffer only short-term symptoms such as high fever, severe headache, stiffness, nausea, abdominal pain and diarrhea, Listeria infection can cause miscarriages and stillbirths among pregnant women.
Source: Food and Drug Administration - Category: Food Science Source Type: news
[UNFPA in WCA] Dakar, Senegal -- 14 December, 2018. The Ouagadougou Partnership Coordination Unit (UCPO) organized its seventh annual meeting from December 12 to 14, 2018, in Dakar, Senegal. The civil society organizations of the nine (9) West African countries (CROSPF) gathered around the Ouagadougou Partnership (PO) met to assess their contribution and to set prospects in the fight for the capture of the demographic dividend in West Africa. They shared good practices and positive experiences as well as the challen
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
Conclusions/SignificanceThese preliminary results suggest the potential utility of CSF metabolomics as a rapid screening test to enhance diagnostic accuracies and improve patient outcomes.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
We report on five cases of patients with a diagnosis of neuroborreliosis based on clinical symptoms, serologic tests and MR imaging results. However, neither of them fulfils the diagnostic criteria for definite neuroborreliosis. Are the diagnostic criteria still valid or is there a need to revis e them? Is our diagnosis correct? Are these cases post-Lyme auto-immune neuronal inflammation, and not due to still active spirochetal infection? Do we need to consider immunosuppressive therapy instead of third-generation cephalosporins?
Source: Acta Neurologica Belgica - Category: Neurology Source Type: research
Sunil KumarIndian Journal of Occupational and Environmental Medicine 2018 22(3):128-137 Lead is a heavy metal. It is used in lead-acid battery, as a coloring agent, paints, and metal alloyed as shielding materials, smelters, printing press, and so on. It is a toxic metal affecting various organs, and developing fetus and young children are more vulnerable to toxicity of lead. This overview is based on the information of toxic potential of lead to human reproduction and reproductive outcome. Exposure to lead may affect libido, semen quality by declining sperm count, motility, viability, integrity, elevation in morphologica...
Source: Indian Journal of Occupational and Environmental Medicine - Category: Occupational Health Authors: Source Type: research
CONCLUSIONS: Alcohol consumption patterns varied widely across settings and binge drinking was more frequent in HIV-positive individuals compared to the general population. Interventions to reduce excessive alcohol use are urgently needed to optimize adherence in the era of universal ART. PMID: 30549445 [PubMed - in process]
Source: Journal of the International AIDS Society - Category: Infectious Diseases Tags: J Int AIDS Soc Source Type: research
Publication date: Available online 14 December 2018Source: Ticks and Tick-borne DiseasesAuthor(s): George Psevdos, Teresa Khoo, Robert Chow, Christopher L Romano, Scott CampbellAbstractIn North America, Lyme disease (LD) is caused predominantly by the spirochete Borrelia burgdorferisensu stricto, and is transmitted by blacklegged ticks. Long Island, New York, is highly endemic for the disease. The C6 peptide (C6P) is currently used as a screening test for LD in our institution. Our objective was to examine how screening with C6P concorded with diagnosis of LD at the Veterans Affairs Medical Center, Northport, Long Island. ...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 15 December 2018Source: Journal of Minimally Invasive GynecologyAuthor(s): Huiying Zhang, Huixia Zhang, Xueqing He, Wenyan Tian, Xueru SongABSTRACTEndometrial polyps are frequently encountered in the uterine cavity of infertile women. There is much debate regarding the treatment of endometrial polyps in patients who are undergoing assisted reproductive technology (ART). A systematic review was performed by searching PubMed, Embase, and the Cochrane Library for retrospective or prospective studies that compared the effect of hysteroscopic resection of polyps with no treatment on pregnancy ...
Source: Journal of Minimally Invasive Gynecology - Category: OBGYN Source Type: research
ConclusionsSelection of a single blastocyst based on information derived from time-lapse monitoring can aid embryo selection for SET.
Source: European Journal of Obstetrics and Gynecology and Reproductive Biology - Category: OBGYN Source Type: research
This study aimed to describe the genome and phenotypic characteristics of O. timonensis Marseille-P3532T and O. senegalensis Marseille-P3587T. The genomes are 4,485,335 bp long for O. timonensis and 4,300,331 bp for O. senegalensis with 38.78% and 36.92% G+C content, respectively. They contain 4306 and 3979 protein-coding and 87 and 273 RNAs genes, respectively. PMID: 30554381 [PubMed - as supplied by publisher]
Source: Antonie van Leeuwenhoek - Category: Microbiology Authors: Tags: Antonie Van Leeuwenhoek Source Type: research
More News: Borrelia | Lyme Disease | Miscarriage | Senegal Health | Tropical Medicine