Multiplexed High-Resolution Melting Assay for Versatile Sample Tracking in a Diagnostic and Research Setting
Modern experimental procedures in molecular genetics, such as next-generation sequencing experiments, require that samples are taken along a whole series of wet- and dry-laboratory steps. It generally is accepted that by increasing the complexity and number of steps in the experimental pipeline, the risk of sample swaps increases. It therefore is recommended to confirm the identity of each individual sample at the end of any pipeline. Here, we present a versatile assay to determine the identity of samples rapidly and efficiently by genotyping 21 single-nucleotide polymorphisms (SNPs) using multiplex high-resolution melting.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: CĂ©line Helsmoortel, R. Frank Kooy, Geert Vandeweyer Tags: Regular Article Source Type: research