Hepatitis C Virus RNA Real-Time Quantitative RT-PCR Method Based on a New Primer Design Strategy

Viral nucleic acids are unstable when improperly collected, handled, and stored, resulting in decreased sensitivity of currently available commercial quantitative nucleic acid testing kits. Using known unstable hepatitis C virus RNA, we developed a real-time quantitative RT-PCR method based on a new primer design strategy to reduce the impact of nucleic acid instability on nucleic acid testing. The performance of the method was evaluated for linearity, limit of detection, precision, specificity, and agreement with commercial hepatitis C virus assays.

Source: Journal of Molecular Diagnostics - November 20, 2015 Category: Pathology Authors: Lida Chen, Wenli Li, Kuo Zhang, Rui Zhang, Tian Lu, Mingju Hao, Tingting Jia, Yu Sun, Guigao Lin, Lunan Wang, Jinming Li Tags: Regular Article Source Type: research