Human Fecal Source Identification with Real-Time Quantitative PCR
We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news
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