Human Fecal Source Identification with Real-Time Quantitative PCR

We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated Bacteroides dorei for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing control, qPCR amplification with an internal amplification control, and quality control data analysis.
Source: Springer protocols feed by Microbiology - Category: Microbiology Source Type: news