Development of a Multiantigen Panel for Improved Detection of Borrelia burgdorferi Infection in Early Lyme Disease Immunoassays

We examined novel and established antigen markers to develop a multiplex panel that identifies early infection using the combined sensitivity of multiple markers while simultaneously maintaining high specificity by requiring positive results for two markers to designate a positive test. Ten markers were selected from our initial analysis of 62 B. burgdorferi surface proteins and synthetic peptides by assessing binding of IgG and IgM to each in a training set of Lyme disease patient samples and controls. In a validation set, this 10-antigen panel identified a higher proportion of early-Lyme-disease patients as positive at the baseline or posttreatment visit than two-tiered testing (87.5% and 67.5%, respectively; P
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research

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In this study, we performed shotgun RNA sequencing on 112 individual adult I. scapularis collected in Wisconsin, USA. We characterized the abundance, prevalence and co-infection rates of viruses, bacteria and eukaryotic microorganisms. We identified pairs of tick-infecting microorganisms whose observed co-infection rates were higher or lower than would be expected, or whose RNA levels were positively correlated in co-infected ticks. Many of these co-occurrence and correlation relationships involved two bunyaviruses, South Bay virus and blacklegged tick phlebovirus-1. These viruses were also the most prevalent microorganism...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
Publication date: Available online 20 July 2018Source: Ticks and Tick-borne DiseasesAuthor(s): Tammi L. Johnson, Christine B. Graham, Sarah E. Maes, Andrias Hojgaard, Amy Fleshman, Karen A. Boegler, Mark J. Delory, Kimetha S. Slater, Sandor E. Karpathy, Jenna K. Bjork, David F. Neitzel, Elizabeth K. Schiffman, Rebecca J. EisenAbstractIn the north-central United States, the blacklegged tick (Ixodes scapularis) is currently known to vector seven human pathogens. These include five bacteria (Borrelia burgdorferi sensu stricto, Borrelia mayonii, Borrelia miyamotoi, Anaplasma phagocytophilum, Ehrlichia muris eauclairensis), one...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
In 1977, a seminal paper described what was subsequently called Lyme arthritis1. The name “Lyme” originated from the initial investigation of the disease that was conducted in the Connecticut towns of Lyme, Old Lyme and East Haddam1. As more of the clinical manifestations of this illness were discovered, the condition became referred to as Lyme disease2-4. In 1983, the principal etio logic agent for Lyme disease in North America was identified in patients3,5. The etiologic agent became known as Borrelia burgdorferi and until fairly recently was the only species of Lyme borrelia identified to cause Lyme disease ...
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: Commentary Source Type: research
Given the variable clinical signs attributed to Borrelia burgdorferi, including infectious arthritis, neurologic disease, and behavioral changes, B burgdorferi is an important differential for decreased performance in sport horses. The primary vectors (Ixodes tick species) are expanding their range and thus Borrelia species are located in a wider area, making exposure more likely. Due to regionally high seroprevalence and vague clinical signs, diagnosis of Lyme disease in the horse is believed overestimated. Antibiotics are first-line treatment of confirmed Lyme disease. A single positive serologic test, by itself, is not ...
Source: Veterinary Clinics of North America: Equine Practice - Category: Veterinary Research Authors: Source Type: research
Publication date: Available online 10 July 2018Source: Ticks and Tick-borne DiseasesAuthor(s): Nicole E. Breuner, Andrias Hojgaard, Adam J. Replogle, Karen A. Boegler, Lars EisenAbstractThe relapsing fever spirochete, Borrelia miyamotoi, is increasingly recognized as a cause of human illness (hard tick-borne relapsing fever) in the United States. We previously demonstrated that single nymphs of the blacklegged tick, Ixodes scapularis, can transmit B. miyamotoi to experimental hosts. However, two recent epidemiological studies from the Northeastern United States indicate that human cases of hard tick-borne relapsing fever p...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Jessica L. Kostick-Dunn, Jerilyn R. Izac, John C. Freedman, Lee T. Szkotnicki, Lee D. Oliver, Richard T. Marconi
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
ConclusionsNon-specific symptoms after tick bite may be caused by uncommon pathogens or co-infection, therefore it should be considered in differential diagnosis after tick bite.
Source: Advances in Medical Sciences - Category: Biomedical Science Source Type: research
Molecular Microbiology,Volume 0, Issue ja, -Not available-.
Source: Molecular Microbiology - Category: Microbiology Authors: Source Type: research
This study shows that humus type can be indicative of nymph abundance. Three types of humus were observed: (1) moder, (2) mull, and (3) mull-moder humus. One of them, moder humus, which is characterized by a thick layer of fragmented leaves, was found in multivariate analyses to be strongly associated with the nymph abundance. This study demonstrates that factors such as saturation deficit do not suffice to explain the differences in nymph abundance among sites. The composition of the soil and especially the type of humus should also be taken into consideration when assessing acarological risk.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
This study suggests that accounting for landscape connectivity may improve model-based predictions of spatial spread patterns of B. burgdorferi s.s. The findings are consistent with possible past dispersal patterns of B. burgdorferi s.s. as determined by phylogeographic studies.
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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