Evaluation of compound activity against hepatitis C virus in replicon systems.

Evaluation of compound activity against hepatitis C virus in replicon systems. Curr Protoc Pharmacol. 2010 Sep;Chapter 13:Unit 13B.1 Authors: Yang G, Huang M Abstract Described in this unit are protocols for evaluation of new chemical entities for activity against hepatitis C virus (HCV) using HCV replicon systems. While agents designed to target NS3 protease, NS3 helicase, and NS5B RNA polymerase can be evaluated in biochemical assays, it is important to confirm their inhibitory effect on HCV RNA replication using HCV replicon systems, especially since replication involves many components besides these enzymes. Screening of compound libraries using replicon systems has led to the discovery of replication inhibitors, which act through different viral targets. PMID: 22294366 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Pharmacology - Category: Drugs & Pharmacology Tags: Curr Protoc Pharmacol Source Type: research