FT-IR Spectroscopic Analysis of Holoptelea integrifolia (Roxb.) Planch Seed Extracts and their Antibacterial Activity

This study was designed to find out the bioactive functional groups present in seed extracts of Holoptelea integrifolia for their antibacterial activity. The characterization of functional groups was achieved by Fourier Transform Infrared Spectroscopy (FT-IR) analysis. Antibacterial activity of methanolic, chloroform and aqueous seed extracts was evaluated against four pathogenic bacterial strains viz. Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhimurium by disc diffusion method. The FT-IR analysis revealed the presence of phenols, alcohols, aldehydes, amines, phosphines, nitriles, aliphatic compounds, azides, esters, β- lactones, amino acids and carboxylic acids etc. Major peaks were observed at 1650-1870 and 3400-3700 cm–1 which correspond to C=O and -OH functional groups, respectively. Among the three seed extracts tested, the methanolic extract showed best antibacterial activity against four pathogenic test pathogens. Staphylococcus aureus was the most sensitive test organism to seed extract of H. integrifolia. The seed extracts have promising antibacterial potential and could be effective natural medicine for the treatment of diseases associated with these pathogenic bacteria. However, further investigation should be done on them to be incorporated in medical applications.
Source: Research Journal of Medicinal Plant - Category: Complementary Medicine Source Type: research