Culture-dependent assessment of bacterial diversity from human milk with lactational mastitis

The objective of this study was to explore culturable bacterial diversity present in the breast milk of mastitis-suffering women by 16S rDNA sequencing and evaluate its antibiotic susceptibility. The culturable bacteria present in the breast milk of 32 women with lactational mastitis were studied. Thirty-seven bacterial isolates comprised of distinct colony characteristics were checked for antibiotic susceptibility and identified by 16S rDNA sequencing. Sequencing of 16S rDNA and BLAST analysis revealed the presence of 17 genera and 30 different species indicating highly diverse community in mastitis milk. The Proteobacteria (51.35 % of total clones) and Firmicutes (37.83 %) were the most abundant phyla and included representatives from the class Gammaproteobacteria, Alphaproteobacteria, and Bacilli. Pseudomonas (6 species) and Staphylococcus (4 species) were the most prevalent genera while Brevundimonas, Bacillus, Enterococcus, Micrococcus, and Macrococcus were infrequently isolated. Staphylococcus sp. (87.5 % of mastitis samples), Staphylococcus aureus (75 %), Pseudomonas aeruginosa (53.12 %), Klebsiella pneumoniae (43.75 %), and Brevundimonas diminuta (37.5 %) were frequently isolated from mastitis milk. Antibiotics like chloramphenicol, gentamicin, ofloxacin, and ciprofloxacin proved to be most effective against mastitis pathogens. Overall analysis suggested that high bacterial diversity in diseased condition could be the result of colon...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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DiscussionRoutine hygienic maintenance of the PTS makes pathogen transmission by PTS highly unlikely, although entry racks should be regularly disinfected. Any involvement of the PTS in our VRE outbreak was also unlikely.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
In this study, MC3T3-E1 cells cultured on the plastic, Ti-6Al-4V, and the cerium oxide–incorporated calcium silicate coatings (CeO2-CS) coating served as the blank, control, and CeO2-CS groups, respectively. A cell counting kit-8 (CCK-8) and flow cytometry were used to evaluate the biocompatibility. The osteoblastic differentiation of the MC3T3-E1 cells was also analyzed by quantitative real-time polymerase chain reaction analysis. The CCK-8 and counts of colony-forming units (CFUs) were used to detect the antibacterial activity of the coating on Enterococcus faecalis. The study showed that the cerium oxide–inc...
Source: Implant Dentistry - Category: Dentistry Tags: Basic and Clinical Research Source Type: research
Publication date: March 2020Source: Materials Science and Engineering: C, Volume 108Author(s): Xiaolin Nie, Shuanglin Wu, Alfred Mensah, Keyu Lu, Qufu WeiAbstractFaced with the emergence and proliferation of antibiotic resistant pathogens, novel nonspecific materials and approaches are required. Herein, we employed electrospinning technology to fabricate nanofibers with antibacterial photodynamic inactivation. This material combines polyacrylonitrile, as a photostable polymer, and biocompatible carbon quantum dots. The resulted nanofibers were successfully characterized by physical and spectroscopic methods. The microbicid...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
Conclusion The results of our study demonstrate that robenidine is not suitable as a sole antimicrobial agent for the treatment of Gram-negative pathogen infections due to the lack of activity against the majority of Gram-negative isolates except for A. baumannii and A. calcoaceticus. However, we demonstrated in vitro efficacy against all selected Gram-negative organisms when robenidine was tested in combination with EDTA or PMBN, including against multidrug-resistant strains. Therefore, robenidine may be an appropriate candidate as a component of a combination preparation for the treatment of otitis externa in dogs. This...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, eight phages were sequentially separated from sewage (Table 2). Phages vB_EcoS_IME18 (IME18), vB_EcoS_IME253 (IME253), vB_EcoM_IME281 (IME281), vB_EcoM_IME338 (IME338), vB_EcoM_IME339 (IME339), vB_EcoM_IME340 (IME340), vB_EcoM_IME341 (IME341), and vB_EcoS_IME347 (IME347) formed clear, bright plaques on soft agar plates (Supplementary Figure S2). TEM imaging showed that phages IME281 (Figure 1), IME339 (Figure 2), IME340 (Figure 3), and IME341 (Figure 4) had a prolate head and a tail with a contractile sheath. The elongated icosahedral head was ∼100 nm long and 80 nm wide, while the tail tube was ∼100...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion This study revealed that Enterococcus species with biofilm potentials and extracellular virulence properties extensively occur in retail RTE shrimps. A significant number of isolated strains are resistant to antibiotics and harbor resistant and virulent genes, denoting a significant route of resistance and virulence dissemination to bacteria in humans. There is an inadequate understanding of the intricacies of antibiotic-resistant enterococci of food origin that belong to enterococci aside from E. faecium and E. faecalis. Findings from this study reveal detailed antibiotic resistance of E. durans, E. casselifla...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
François Chassagne1†, Xinyi Huang1†, James T. Lyles1 and Cassandra L. Quave1,2* 1Center for the Study of Human Health, Emory University, Atlanta, GA, United States 2Department of Dermatology, Emory University, Atlanta, GA, United States In the search for new therapeutic solutions to address an increasing number of multidrug-resistant bacterial pathogens, secondary metabolites from plants have proven to be a rich source of antimicrobial compounds. Ginkgo biloba, a tree native to China, has been spread around the world as an ornamental tree. Its seeds have been used as snacks and medical mater...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion Genomic analyses herein performed allowed us to confirm the recently (Pérez-Cataluña et al., 2018a,b) suggested amendment of A. butzleri as Al. butzlerii, comb. nov. Antimicrobial susceptibility tests defined Ab 55 and Ab 6V strains as resistant to vancomycin, tetracyclin, nalidixic acid (only Ab 55 whereas Ab 6V is intermediate resistant), erythromycin, and β-lactam antibiotics. Moreover, in our strains isolated from shellfish, we identified numerous virulence, antibiotic, and heavy metal resistance determinants, also additional to those previously found in the genome sequenced A. butzleri ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: August 2016 Source:Small Ruminant Research, Volume 141 Author(s): Simone Dore, Manuele Liciardi, Simonetta Amatiste, Stefania Bergagna, Giuseppe Bolzoni, Vincenzo Caligiuri, Anna Cerrone, Giovanni Farina, Cosimo Oscar Montagna, Maria Antonietta Saletti, Maria Luisa Scatassa, Giovanni Sotgiu, Eugenia Agnese Cannas Mastitis is the most important disease of dairy small ruminants affecting animal welfare, agricultural economy, and food safety. Only a few investigations on the bacterial epidemiology of udder infections have been performed. Aim of the study was to describe the Italian epidemiolog...
Source: Small Ruminant Research - Category: Zoology Source Type: research
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