From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review.

From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review.
Comb Chem High Throughput Screen. 2015 Nov 10;
Authors: Ramírez-Velasco L, Armendáriz-Ruiz M, Rodríguez-González JA, Müller-Santos M, Asaff-Torres A, Mateos-Díaz JC
Abstract
Feruloyl esterases (FAEs) are a diverse group of hydrolases widely distributed in plants and microorganisms which catalyzes the cleavage and formation of ester bonds between plant cell wall polysaccharides and phenolic acids. FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of substrates for cleaving ester bonds and synthesizing high-added value molecules through esterification and transesterification reactions. During the past two decades extensive studies have been carried out on the production, characterization and classification of FAEs, however only a few reports of suitable High Throughput Screening assays for this kind of enzymes have been reported. This review is focused on a concise but complete revision of classical to High Throughput Screening methods for FAEs, highlighting its advantages and disadvantages, and finally suggesting future perspectives for this important research field.
PMID: 26552434 [PubMed - as supplied by publisher]

Source: Combinatorial Chemistry and High Throughput Screening - November 10, 2015 Category: Chemistry Authors: Ramírez-Velasco L, Armendáriz-Ruiz M, Rodríguez-González JA, Müller-Santos M, Asaff-Torres A, Mateos-Díaz JC Tags: Comb Chem High Throughput Screen Source Type: research