Lactobacillus plantarum LB95 impairs the virulence potential of gram-positive and gram-negative food borne pathogens in HT-29 and Vero cell cultures.

Lactobacillus plantarum LB95 impairs the virulence potential of gram-positive and gram-negative food borne pathogens in HT-29 and Vero cell cultures. J Med Microbiol. 2015 Oct 27; Authors: Dutra V, Silva AC, Cabrita P, Peres C, Malcata X, Brito L Abstract Listeria monocytogenes, Salmonella enterica and verocytotoxigenic Escherichia coli (VTEC) are among the most important agents responsible for food outbreaks occurred worldwide. In this work, two Lactobacillus spp. strains (LABs), Lactobacillus plantarum (LB95) and Lactobacillus paraplantarum (LB13) previously isolated from spontaneously fermenting olive brines, and two reference probiotic strains, Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, were investigated for their abilities to attenuate the virulence of the aforementioned pathogens using animal cell culture assays. In competitive exclusion assays, the relative percentages of adhesion and invasion of S. Enteritidis were significantly reduced when the human HT-29 cell line was previously exposed to LB95. The relative percentage of invasion of L. monocytogenes was significantly reduced when the HT-29 cells were previously exposed to LB95. In the cytotoxicity assays, the cell-free supernatant of the co-culture (CFSC) of VTEC with LB95 accounted for the lowest value obtained among the co-cultures of the VTEC with the LABs, and was significantly lower than the value obtained with the co-culture of the VTEC with the two...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research