Improved blood culture identification by FilmArray in cultures from regional hospitals compared to teaching hospital cultures.

Improved blood culture identification by FilmArray in cultures from regional hospitals compared to teaching hospital cultures. J Med Microbiol. 2015 Oct 26; Authors: Inglis TJ, Bzdyl N, Chua IJ, Urosevic NM, Leung MJ, Geelhoed E Abstract Rapid identification of bacteria isolated from blood cultures by direct MALDI-TOF mass spectrometer is now in widespread use in major centres but is not yet feasible in smaller hospital laboratories. A FilmArray multiplex PCR panel for identification of blood culture isolates (BCID) provides an alternative approach to near point-of-care microbial identification in regional hospitals. We assessed the accuracy and time to identification of the BCID FilmArray in a consecutive series of 149 blood cultures from 143 patients in teaching hospital and smaller regional hospitals, currently identified by direct MALDI-TOF and proprietary molecular methods. The BCID FilmArray contained 18 of 34 species and 20 of 23 species isolated from teaching and regional hospital respectively. 85% teaching hospital and 100% regional hospital monomicrobial blood cultures were identified, compared with 60 and 68% respectively for direct MALDI-TOF on the same cultures. There were no incorrect results from blood cultures containing <italic>Staphylococcus aureus</i>, streptococci, <italic>Pseudomonas aeruginosa</i> or Enterobacteriaceae. The three discrepant results were all in mixed cultures. The mean red...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research