Establishment of a Primary Screening Assay for the DHX9 Helicase.
Establishment of a Primary Screening Assay for the DHX9 Helicase. Comb Chem High Throughput Screen. 2015 Oct 18; Authors: Cencic R, Sénéchal P, Pelletier J Abstract The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional control and translation regulation. Its association with the EWS-FLI1 fusion product, as well as the finding that its suppression can be synthetic lethal with the BCL-2 family inhibitor ABT-737 indicates a potential role in tumor maintenance. Hence, to identify small molecules that could interfere with its activity, we developed a homogenous RNA-dependent ATPase assay. We show that aurintricarboxylic acid, a promiscuous protein-nucleic acid inhibitor prevents DHX9-mediated hydrolysis demonstrating that the assay is also capable of detecting compounds that impinge on DHX9:RNA association. PMID: 26477352 [PubMed - as supplied by publisher]
DiscussionThe interventions of education and medication alerts resulted in a significant increase in the percentage of patients receiving appropriate doses of dilTIAZem and a nonsignificant decrease in the incidence of hypotension. This process-oriented intervention resulted in an improvement in appropriate dilTIAZem doses at our site. Rate control was not statistically significantly different between the 2 groups. Long-term sustainability of this intervention requires further study.
ConclusionsVitamin D insufficiency was prevalent in both newly diagnosed axSpA patients and healthy controls. There was no association between vitamin D and pain and disease activity in the newly diagnosed axSpA patients. Monitoring vitamin D levels is important and early intervention for vitamin D insufficiency is needed, especially in female patients.
ConclusionVacuum assisted dressing is more effective than traditional wound dressing in wound healing of open fractures.
This study aims to reflect on the process of adoption of the preprints publication model, briefly delimiting its history and current use by the national and the international scientific community. Departing from the literature and a consultation of preprint repositories, this reflection intends to highlight the main challenges that preprint implementation will face in the Nursing area, along with its specificities. While considering its benefits for scientific dissemination, this study points out the difficulties that may arise from the implementation of mostly north-American science models in a peripheral country such as ...
RESUMO Objetivo Identificar na literatura a avalia ção da qualidade dos serviços de Atenção Primária à Saúde sob a ótica de cuidadores de crianças por meio da aplicação do PCATool, versão infantil. Método Revisão integrativa realizada nas bases de dados MEDLINE, CINAHL, LILACS, BDENF e Web of Science. Resultados Foram selecionados 17 artigos. Todos os estudos incluídos eram descritivos (100%) e possuíam nível de evidência IV (100%). O componente grau de afiliação (média=7,...
CONCLUSIONS: Neonatal screening with the red reflex is not sufficient to detect intraocular disease. A normal neonatal red reflex does no exclude intraocular disease. It has a specificity of nearly 90%, but its sensitivity is only 56%. Most retinal haemorrhages are undiagnosed as they cannot be detected with a red reflex. PMID: 31826811 [PubMed - as supplied by publisher]
Reaction to claims of study misconduct have dominated the press and social media since the report dropped earlier this week.Medscape Medical News
At least 1,300 people have died from the flu so far this season, according to a preliminary estimate released Friday by the US Centers for Disease Control and Prevention.
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There is evidence that an ancient Chinese form of martial arts improves your mind and body, and may even contribute to a longer life.