The contribution of aerobic and anaerobic respiration to intestinal colonization and virulence for Salmonella typhimurium in the chicken.

The contribution of aerobic and anaerobic respiration to intestinal colonization and virulence for Salmonella typhimurium in the chicken. Avian Pathol. 2015 Oct;44(5):401-7 Authors: Barrow PA, Berchieri A, Freitas Neto OC, Lovell M Abstract The basic mechanism whereby Salmonella serovars colonize the chicken intestine remains poorly understood. Previous studies have indicated that proton-translocating proteins utilizing oxygen as terminal electron acceptor do not appear to be of major importance in the gut of the newly hatched chicken and consequently they would be even less significant during intestinal colonization of more mature chickens where the complex gut microflora would trap most of the oxygen in the lumen. Consequently, alternative electron acceptors may be more significant or, in their absence, substrate-level phosphorylation may also be important to Salmonella serovars in this environment. To investigate this we constructed mutants of Salmonella enterica serovar Typhimurium defective in various aspects of oxidative or substrate-level phosphorylation to assess their role in colonization of the chicken intestine, assessed through faecal shedding, and virulence. Mutations affecting use of oxygen or alternative electron acceptors did not eliminate faecal shedding. By contrast mutations in either pta (phosphotransacetylase) or ackA (acetate kinase) abolished shedding. The pta but not the ackA mutation also abolished systemic virulence for chickens...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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Publication date: Available online 12 November 2019Source: Veterinary MicrobiologyAuthor(s): Sahar Rostami, Jalil Mehrzad, Ramak Yahyaraeyat, Taghi Zahraei SalehiAbstractSalmonella enterica Subsp enterica serovar Typhimurium (S. Typhimurium, ST) is one of the most important serovars of the genus Salmonella in human and animals. Because of its intracellular tropism, monocytes/macrophages are pivotal in killing of Salmonella serovars; they are also responsible for transporting of ST to extra-intestinal organs. To investigate the effect of the ST on the functions of avian innate immune cells, almost homogeneous enriched monoc...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 4 November 2019Source: Preventive Veterinary MedicineAuthor(s): Carla Correia-Gomes, Nick SparksAbstractBackyard poultry producers have been associated with outbreaks of exotic (e.g. avian influenza) and endemic (e.g. Salmonella) disease all over the world. Currently in the UK the registration of small flocks (less than 50 birds) with local authorities is voluntary therefore there is not an accurate record of how many keepers and birds there are or where they are located. This lack of information (e.g. how many birds they keep, what type of birds, biosecurity measures they implement, etc....
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
IJERPH, Vol. 16, Pages 4235: Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types International Journal of Environmental Research and Public Health doi: 10.3390/ijerph16214235 Authors: Aung Chen Chau Yap Lim Humaidi Chua Yeo Yap Oh Manogaran Hapuarachchi Maiwald Tee Barkham Koh Gutiérrez Schlundt Ng Non-typhoidal salmonellosis is a leading cause of foodborne zoonosis. To better understand the epidemiology of human salmonellosis, this study aimed to determine the prevalence, antimicrobial res...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
The follicle-associated epithelium (FAE) is a specialized structure that samples luminal antigens and transports them into mucosa-associated lymphoid tissues (MALT). In mammals, transcytosis of antigens across the gut epithelium is performed by a subset of FAE cells known as M cells. Here we show that colony-stimulating factor 1 receptor (CSF1R) is expressed by a subset of cells in the avian bursa of Fabricius FAE. Expression was initially detected using a CSF1R-reporter transgene that also label subsets of bursal macrophages. Immunohistochemical detection using a specific monoclonal antibody confirmed abundant expression ...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Marc. K. Kouam, Herman M.F Biekop, Bridget Katte, Alexis TeguiaAbstractSalmonella infections in poultry farms are overlooked in many African countries; yet these infections are mostly zoonotic with impact on both poultry industry and public health. Considering the impact of Salmonella in laying hens, and the role of laying hens as a source of Salmonella outbreak in human, knowledge of the status of Salmonella on laying hen farms as well as the factors influencing the presence of Salmonella is imp...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
Abstract Salmonella infections in poultry farms are overlooked in many African countries; yet these infections are mostly zoonotic with impact on both poultry industry and public health. Considering the impact of Salmonella in laying hens, and the role of laying hens as a source of Salmonella outbreak in human, knowledge of the status of Salmonella on laying hen farms as well as the factors influencing the presence of Salmonella is important. In a cross sectional study, cloacal swabs were collected from 270 commercial laying hens on 27 farms located in Menoua Division. These samples were cultured on standard media...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Publication date: Available online 18 September 2019Source: Preventive Veterinary MedicineAuthor(s): Marisa Peyre, Linda Hoinville, James Njoroge, Angus Cameron, Daniel Traon, Flavie Goutard, Clémentine Calba, Vladimir Grosbois, Alexis Delabouglise, Viktor Varant, Julian Drewe, Dirk Pfeiffer, Barbara HäslerAbstractInformation about infectious diseases at the global level relies on effective, efficient and sustainable national and international surveillance systems. Surveillance systems need to be regularly evaluated to ensure their effectiveness, the quality of the data and information provided, as well as to b...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
by Lizeth Lacharme-Lora, Si ân V. Owen, Richard Blundell, Rocío Canals, Nicolas Wenner, Blanca Perez-Sepulveda, Wai Yee Fong, Rachel Gilroy, Paul Wigley, Jay C. D. Hinton Over recent decades,Salmonella infection research has predominantly relied on murine infection models. However, in many cases the infection phenotypes ofSalmonella pathovars in mice do not recapitulate human disease. For example,Salmonella Typhimurium ST313 is associated with enhanced invasive infection of immunocompromised people in Africa, but infection of mice and other animal models with ST313 have not consistently reproduced this invasi...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
This study was undertaken to compare the expression of interferon-gamma (IFN-γ) in chickens generated by different vaccine formulations. Eighty day-old Lohmann layer chicks were divided into four groups of twenty birds each for the experiment. This comprised a non-vaccinated negative control group (NEG), a group vaccinated with the live 9R vaccine by the injection route (SC), a group vaccinated with alginate-coated chitosan microparticles encapsulating live plasmid-cured S. Gallinarum strain 9 (PC) by the oral route and a group vaccinated with a weak attenuated live S. Gallinarum strain 9 encapsulated in alginate-coa...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
In this study, room temperature (RT) of 25°C was considered as control, and RT at 32°C for 8 h per day was set as the HS treatment. After 3 weeks, the intestinal contents of jejunum, ileum, and cecum were harvested to investigate the microbiota composition variations by 16S ribosomal RNA amplicon sequencing. And the weight gain, adipose indices, intestinal morphology, and a certain number of serum biochemical parameters were also measured and analyzed. The results showed the microbial species at different levels differentially enriched in duck jejunum and cecum under HS, while no significant data were observed in i...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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