Efficacy of the FilmArray Blood Culture Identification panel for direct molecular diagnosis of infectious diseases from samples other than blood.

Efficacy of the FilmArray Blood Culture Identification panel for direct molecular diagnosis of infectious diseases from samples other than blood. J Med Microbiol. 2015 Oct 1; Authors: Micó M, Navarro F, de Miniac D, González Y, Brell A, López C, Sánchez-Reus F, Mirelis B, Coll P Abstract Molecular-based techniques reduce the delay in diagnosing infectious diseases and therefore contribute to better patient outcomes. We assessed the FilmArray Blood Culture Identification (Biofire Diagnostics Inc. bioMérieux) panel directly on clinical specimens other than blood: cerebrospinal, joint, pleural and ascitic fluids, bronchoscopy samples, and abscesses. We compared the results from 88 samples obtained by culture-based techniques. The percentage of agreement between the two methods was 75% with a Cohen's kappa of 0.51. Global sensitivity and specificity using the FilmArray Blood Culture Identification (BCID) panel were 71% and 97%, respectively. Sensitivity was poorer in samples with a low bacterial load, such as ascitic and pleural fluids (25%), whereas sensitivity for abscess samples was high (89%). These findings suggest that the FilmArray BCID panel could be useful to perform microbiological diagnosis directly from samples other than positive blood cultures, as it offers acceptable sensitivity and moderate agreement with conventional microbiological methods. Nevertheless, cost-benefit studies should be performed before introducing t...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research