Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, cecal tonsils, and spleen of Salmonella-challenged broilers.

Modulation of cytokine gene expression by selected Lactobacillus isolates in the ileum, cecal tonsils, and spleen of Salmonella-challenged broilers. Avian Pathol. 2015 Sep 22;:1-31 Authors: Hu JL, Yu H, Kulkarni RR, Sharif S, Cui SW, Xie MY, Nie SP, Gong J Abstract Probiotics have been used to control Salmonella colonization in the chicken intestine. Recently, we demonstrated that certain selected Lactobacillus isolates were able to reduce Salmonella infection in the chicken spleen and liver as well as down-regulated Salmonella pathogenicity island 1 (SPI-1) virulence gene expression in the chicken cecum. To further understand the mechanisms through which Lactobacillus protected chickens from Salmonella infection, the present study has investigated the Lactobacillus isolate(s)-induced host immune response of chickens to Salmonella infection. A thorough examination of cytokine gene expression in the ileum, cecal tonsils and spleen on days 1 and 3 post Salmonella infection showed a dynamic spatial and temporal response to Salmonella infection and Lactobacillus treatments. In most instances, it was evident that treatment of chickens with Lactobacillus isolates could significantly attenuate Salmonella-induced changes in the gene expression profile. These included the genes encoding pro-inflammatory cytokines [lipopolysaccharide-induced TNF factor (LITAF), interleukin (IL)-6, and IL-8], T helper (Th) 1 cytokines [IL-12 and interferon (IFN...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research