Efficacy of Lepidium sativum against carbon tetra chloride induced hepatotoxicity and determination of its bioactive compounds by GC-MS

Publication date: Available online 25 September 2015 Source:Toxicology Reports Author(s): Abdulrahman Khazim Al-Asmari, Md Tanwir Athar, Hamoud M. Al-Shahrani, Saleh Ibrahim Al-Dakheel, Mohammed A. Al-Ghmadi Lepidium sativum seed (LSS) (family Cruciferae) has been used in traditional medicine for the treatment of jaundice, liver problems, spleen diseases and gastrointestinal disorders. It was also reported to possess antihypertensive, diuretic, anti-asthmatic, antioxidant, and anti-inflammatory activities. Attempt has been made to study hepatoprotective potential of LSS available in Saudi Arabian Market. The aim of the present study was to determine the hepatoprotective effect of ethanolic extracts of LSS against carbon tetrachloride (CCl4) induced acute liver injury in rats. The bioactive compounds responsible for this activity have been analyzed by GC-MS. To evaluate the hepatoprotective activity, Six groups (n = 6) of rats were taken. First group was control, second was toxic and other groups received oral test solutions: 100mg/kg silymarin, or LSS (100, 200, and 400mg/kg), once daily for 7 consecutive days, followed by hepatotoxicity induction with CCl4. Blood and liver tissues were collected for biochemical, antioxidant and microscopic analyses. The bioactive constituents present in the extract were analyzed by GC-MS. Results showed that pretreatment with LSS and silymarin significantly reduced the level of serum alanine transaminase (ALT), aspartate trans...
Source: Toxicology Reports - Category: Toxicology Source Type: research